Document Detail


Leukaemia inhibitory factor is necessary for maintenance of haematopoietic stem cells and thymocyte stimulation.
MedLine Citation:
PMID:  8497320     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Leukaemia inhibitory factor (LIF) has a variety of effects on different cell types in vitro, inhibiting the differentiation of embryonic stem cells and promoting the survival and/or proliferation of primitive haematopoietic precursors and primordial germ cells. Here we show that LIF-deficient mice derived by gene targeting techniques have dramatically decreased numbers of stem cells in spleen and bone marrow. Injection of spleen and marrow cells from these mice promotes long-term survival of lethally irradiated wild-type animals, however, showing that the LIF- stem cells remain pluripotent. The numbers of committed progenitors are also reduced in the spleen but not the bone marrow, suggesting that stem cells interact differently with the splenic and medullary microenvironment. Heterozygous animals are intermediate in phenotype, implying that LIF has a dosage effect, and defects in stem cell number can be compensated by exogenous LIF. LIF thus appears to be required for the survival of the normal pool of stem cells, but not their terminal differentiation.
Authors:
J L Escary; J Perreau; D Duménil; S Ezine; P Brûlet
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nature     Volume:  363     ISSN:  0028-0836     ISO Abbreviation:  Nature     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-06-24     Completed Date:  1993-06-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  361-4     Citation Subset:  IM    
Affiliation:
Unité d'Embryologie Moléculaire de l'Institut Pasteur, URA 1148 du CNRS, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
B-Lymphocytes / immunology
Base Sequence
Bone Marrow Cells
Cell Count
Cells, Cultured
Chromosome Mapping
DNA, Single-Stranded
Female
Growth Inhibitors / deficiency,  genetics,  physiology*
Hematopoiesis / genetics
Hematopoietic Stem Cells / cytology,  physiology*
Interleukin-6*
Leukemia Inhibitory Factor
Lymphokines / deficiency,  genetics,  physiology*
Male
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred DBA
Molecular Sequence Data
Spleen / cytology
T-Lymphocytes / immunology
Chemical
Reg. No./Substance:
0/DNA, Single-Stranded; 0/Growth Inhibitors; 0/Interleukin-6; 0/Leukemia Inhibitory Factor; 0/Lif protein, mouse; 0/Lymphokines

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