Document Detail

Leukaemia inhibitory factor in endometrium during the oestrous cycle, early pregnancy and in ovariectomized steroid-treated ewes.
MedLine Citation:
PMID:  9155738     Owner:  NLM     Status:  MEDLINE    
Leukaemia inhibitory factor (LIF), a pleiotropic cytokine, is essential for blastocyst implantation in mice and maintains the development of ovine embryos in culture. The expression of LIF was examined by northern blot analysis in endometrial tissue from cyclic (days 4-16) and pregnant (days 4-20) ewes, and the corresponding protein was immunolocalized. Expression of mRNA encoding LIF remained relatively constant throughout the oestrous cycle and was present during early pregnancy. A decrease in mRNA encoding LIF was observed during early pregnancy (on days 12-14) and expression was highest on days 16-20. Immunoreactive LIF was present in the cellular compartments of the endometrium throughout the oestrous cycle and early pregnancy, with maximal immunostaining in the caruncular and intercaruncular luminal epithelium, and moderate staining in the glandular epithelium and intercaruncular stroma. Immunoreactive LIF was also detected in the trophoblast cells of day 17 blastocysts. Separately cultured endometrial epithelial and stromal cells from pregnant animals both expressed mRNA encoding LIF. Ovariectomized steroid-treated ewes were studied to establish whether steroid hormones had a role in regulating endometrial LIF. Ewes treated with oestradiol alone showed lower concentrations of immunoreactive LIF in the endometrium in comparison to ovariectomized, control animals, while treatment of ovariectomized animals with both oestradiol and progesterone had a greater inhibitory effect on LIF immunolocalization. These studies demonstrate the presence of mRNA encoding LIF and protein throughout the oestrous cycle and early pregnancy and suggest that steroid hormones may be involved in their regulation.
D Vogiagis; R C Fry; R M Sandeman; L A Salamonsen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  109     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-06-12     Completed Date:  1997-06-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  279-88     Citation Subset:  IM    
Prince Henry's Institute of Medical Research, Clayton, Victoria, Australia.
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MeSH Terms
Blotting, Northern
Endometrium / immunology*
Estradiol / pharmacology
Estrus / immunology*
Growth Inhibitors / genetics,  metabolism*
Leukemia Inhibitory Factor
Lymphokines / genetics,  metabolism*
Pregnancy, Animal / immunology*
Progesterone / pharmacology
RNA, Messenger / analysis
Sheep / physiology*
Reg. No./Substance:
0/Growth Inhibitors; 0/Interleukin-6; 0/Leukemia Inhibitory Factor; 0/Lymphokines; 0/RNA, Messenger; 50-28-2/Estradiol; 57-83-0/Progesterone

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