Document Detail

Leucine and methionine deficiency impairs immunity to gastrointestinal parasites during lactation.
MedLine Citation:
PMID:  22571601     Owner:  NLM     Status:  MEDLINE    
Lactating rats reinfected with Nippostrongylus brasiliensis fed low-crude protein (CP) foods show reduced lactational performance and less resistance to parasites compared with their high-CP counterparts. Here, we hypothesised that feeding high-CP foods deficient in specific essential amino acids (AA) would result in similar penalties. Second-parity lactating rats, immunised with 1600 N. brasiliensis infective larvae before mating, were fed foods with either 250 (high protein; HP) or 150 (low protein; LP) g CP/kg, or were HP deficient in either leucine (HP-Leu) or methionine (HP-Met). On day 1 of lactation, litter size was standardised at twelve pups. On day 2, dams were either reinfected with 1600 N. brasiliensis larvae or sham-infected with PBS. Dams and litters were weighed daily until either day 8 or 11, when worm burdens, and inflammatory cells and systemic levels of N. brasiliensis-specific Ig isotypes were assessed. Data from five out of sixteen HP-Met rats were omitted due to very high levels of food refusals from parturition onwards. Relative to feeding HP foods, feeding LP, HP-Met and HP-Leu foods reduced dam weight gain and, to a lesser extent, litter weight gain, and increased the number of worm eggs in the colon, indicative of a reduction in resistance to parasites. However, only feeding LP and HP-Leu foods resulted in increased worm numbers, while none of the feeding treatments affected systemic Ig, mast and goblet cells, and eosinophil numbers. The present results support the view that resistance to parasites during lactation may be sensitive to specific essential AA scarcity.
Panagiotis Sakkas; Leigh A Jones; Jos G M Houdijk; Spiridoula Athanasiadou; Dave P Knox; Ilias Kyriazakis
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-09
Journal Detail:
Title:  The British journal of nutrition     Volume:  109     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-25     Completed Date:  2013-03-19     Revised Date:  2014-10-14    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  273-82     Citation Subset:  IM    
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MeSH Terms
Antibodies, Helminth / analysis
Diet, Protein-Restricted / adverse effects
Feces / parasitology
Gastrointestinal Tract / immunology,  parasitology*,  pathology
Immunity, Mucosal*
Immunocompromised Host*
Lactation / blood,  immunology*
Larva / immunology
Leucine / deficiency*
Maternal Nutritional Physiological Phenomena
Methionine / deficiency*
Nippostrongylus / physiology*
Parasite Load
Random Allocation
Rats, Sprague-Dawley
Strongylida Infections / immunology,  parasitology,  pathology,  prevention & control
Weight Loss
Grant Support
BB/D017890/1//Biotechnology and Biological Sciences Research Council; //Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Antibodies, Helminth; AE28F7PNPL/Methionine; GMW67QNF9C/Leucine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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