| Leucine at position 383 of fusion protein is responsible for fusogenicity of wild-type mumps virus in B95a cells. | |
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MedLine Citation:
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PMID: 20332648 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Mumps virus is isolated in Vero cells and, recently, B95a cells have been reported to be susceptible to it. Currently circulating wild-type mumps virus strains (genotypes B, G, J and L) induced cytopathic effects in both Vero and B95a cells. On the other hand, the Hoshino vaccine strain (KO3) did not induce cytopathic effects in B95a cells. In this study, differences in fusion inducibility were investigated. METHODS: Nucleotide sequences of the fusion (F) and hemagglutinin-neuraminidase (HN) protein regions were compared. The F and HN expression plasmids were constructed and fusion analysis was conducted, using recombinant F expression plasmids under the control of T7 RNA polymerase. RESULTS: Extensive cell fusion was observed when B95a cells were transfected with the wild-type F expression plasmid as the F expression partner; 13-16 amino acid differences were observed in the F protein region between the KO3 and the wild types. F expression plasmids with leucine at position 383 of the F protein induced large cell fusion in B95a cells. CONCLUSION: Leucine at position 383 of the F protein of the wild types was the critical amino acid for fusion inducibility in B95a cells. |
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Authors:
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Naoko Yoshida; Tetsuo Nakayama |
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Publication Detail:
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Type: Journal Article Date: 2010-03-23 |
Journal Detail:
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Title: Intervirology Volume: 53 ISSN: 1423-0100 ISO Abbreviation: Intervirology Publication Date: 2010 |
Date Detail:
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Created Date: 2010-06-10 Completed Date: 2010-09-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364265 Medline TA: Intervirology Country: Switzerland |
Other Details:
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Languages: eng Pagination: 193-202 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Tachikawa Kyousai Hospital, Tachikawa, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Callithrix Cell Line Cercopithecus aethiops Gene Expression Genetic Vectors HN Protein / genetics Leucine / genetics, physiology* Mumps virus / genetics, physiology* Plasmids Recombinant Proteins / genetics, metabolism Sequence Homology, Amino Acid Viral Fusion Proteins / genetics, physiology* Virus Internalization* |
| Chemical | |
Reg. No./Substance:
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0/HN Protein; 0/Recombinant Proteins; 0/Viral Fusion Proteins; 61-90-5/Leucine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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