| A Leu-Leu sequence is essential for COOH-terminal targeting signal of GLUT4 glucose transporter in fibroblasts. | |
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MedLine Citation:
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PMID: 8300557 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the insulin-responsive tissues, muscle and adipose, the GLUT4 glucose transporter isoform accounts for most of the increase in hexose flux in response to hormone. In these cell types, as well as in fibroblasts transfected with cDNAs encoding the transporters, GLUT1 and GLUT4 are sorted to different subcellular locations. In the latter, GLUT1 is found primarily at the cell surface whereas GLUT4 localizes to the interior of the cell in a perinuclear distribution. The construction and analysis of chimeras of these two transporter isoforms have allowed identification of the COOH-terminal 30 amino acids as a critical sorting signal for differential localization of the transporters. In this study, we show that 2 residues present in the GLUT4 COOH terminus, Leu-489 and Leu-490, are critical for the intracellular sequestration of this isoform in NIH3T3 cells. |
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Authors:
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K J Verhey; M J Birnbaum |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 269 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1994 Jan |
Date Detail:
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Created Date: 1994-03-04 Completed Date: 1994-03-04 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2353-6 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Amino Acid Sequence Animals Glucose Transporter Type 1 Glucose Transporter Type 4 Humans Leucine* Mice Molecular Sequence Data Monosaccharide Transport Proteins / biosynthesis*, chemistry Muscle Proteins* Mutagenesis, Site-Directed Point Mutation Protein Conformation Protein Sorting Signals / metabolism* Rats Recombinant Fusion Proteins / biosynthesis Sequence Homology, Amino Acid Transfection |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK39519/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glucose Transporter Type 1; 0/Glucose Transporter Type 4; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins; 0/Protein Sorting Signals; 0/Recombinant Fusion Proteins; 0/SLC2A1 protein, human; 0/SLC2A4 protein, human; 0/Slc2a1 protein, mouse; 0/Slc2a1 protein, rat; 0/Slc2a4 protein, mouse; 0/Slc2a4 protein, rat; 61-90-5/Leucine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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