| Lethality of shock pressures to a marine Vibrio sp. isolated from a ship's ballast water. | |
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MedLine Citation:
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PMID: 17190270 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lethal effects of shock pressure treatment on suspended Vibrio sp. cells were examined. Lethality of shock pressures to the Vibrio sp. cells increased with the increase in the values of maximum shock pressures generated in the cell suspension. When the value was around 114 MPa, the total number of colony-forming cells was reduced from 10(8.5+/-0.1) colony-forming units (CFU) to 10(3.3) - 10(3.4) CFU/ml, and complete loss of colony-forming ability was seen at the maximum value of 282 MPa. Almost all the cells could survive after the exposure to shock pressures including the maximum value of around 189 MPa in the presence of 2% sodium ascorbate (VitC-Na), whereas the total number of colony-forming cells was reduced to 10(1.6)-10(2.1) CFU/ml in the absence of VitC-Na. The surviving cells, however, showed sensitivity to 0.8% sodium cholate, a strong detergent. About 11% of cell-associated proteins had leaked out when the cells were exposed to lethal shock pressures including the maximum value of around 290 MPa in the absence of VitC-Na. These results indicate that the radicals generated in the cell suspension may be closely related to the loss of colony-forming ability of the Vibrio sp. cells. Damage to the outer membrane structure also seems to have occurred by the exposure to shock pressures. |
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Authors:
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Haruo Mimura; Akihisa Abe; Ryo Katakura; Hideki Kawasaki; Kazutoshi Yoshida; Hiroshi Ishida |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biocontrol science Volume: 11 ISSN: 1342-4815 ISO Abbreviation: Biocontrol Sci Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-12-27 Completed Date: 2007-01-18 Revised Date: 2007-06-01 |
Medline Journal Info:
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Nlm Unique ID: 9712121 Medline TA: Biocontrol Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 159-66 Citation Subset: IM |
Affiliation:
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Faculty of Maritime Sciences, Kobe University, 5-1-1, Fukae, Higashinada, Kobe 658-0022, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biodiversity Colony Count, Microbial Escherichia coli / metabolism Hydrostatic Pressure Microscopy, Electron, Scanning Pressure Proteins / chemistry* Seawater / microbiology* Shock Time Factors Travel Vibrio / metabolism* Vibrio cholerae / metabolism Water Microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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