Document Detail


Lethality of shock pressures to a marine Vibrio sp. isolated from a ship's ballast water.
MedLine Citation:
PMID:  17190270     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lethal effects of shock pressure treatment on suspended Vibrio sp. cells were examined. Lethality of shock pressures to the Vibrio sp. cells increased with the increase in the values of maximum shock pressures generated in the cell suspension. When the value was around 114 MPa, the total number of colony-forming cells was reduced from 10(8.5+/-0.1) colony-forming units (CFU) to 10(3.3) - 10(3.4) CFU/ml, and complete loss of colony-forming ability was seen at the maximum value of 282 MPa. Almost all the cells could survive after the exposure to shock pressures including the maximum value of around 189 MPa in the presence of 2% sodium ascorbate (VitC-Na), whereas the total number of colony-forming cells was reduced to 10(1.6)-10(2.1) CFU/ml in the absence of VitC-Na. The surviving cells, however, showed sensitivity to 0.8% sodium cholate, a strong detergent. About 11% of cell-associated proteins had leaked out when the cells were exposed to lethal shock pressures including the maximum value of around 290 MPa in the absence of VitC-Na. These results indicate that the radicals generated in the cell suspension may be closely related to the loss of colony-forming ability of the Vibrio sp. cells. Damage to the outer membrane structure also seems to have occurred by the exposure to shock pressures.
Authors:
Haruo Mimura; Akihisa Abe; Ryo Katakura; Hideki Kawasaki; Kazutoshi Yoshida; Hiroshi Ishida
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biocontrol science     Volume:  11     ISSN:  1342-4815     ISO Abbreviation:  Biocontrol Sci     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-27     Completed Date:  2007-01-18     Revised Date:  2007-06-01    
Medline Journal Info:
Nlm Unique ID:  9712121     Medline TA:  Biocontrol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  159-66     Citation Subset:  IM    
Affiliation:
Faculty of Maritime Sciences, Kobe University, 5-1-1, Fukae, Higashinada, Kobe 658-0022, Japan.
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MeSH Terms
Descriptor/Qualifier:
Biodiversity
Colony Count, Microbial
Escherichia coli / metabolism
Hydrostatic Pressure
Microscopy, Electron, Scanning
Pressure
Proteins / chemistry*
Seawater / microbiology*
Shock
Time Factors
Travel
Vibrio / metabolism*
Vibrio cholerae / metabolism
Water Microbiology*
Chemical
Reg. No./Substance:
0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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