Document Detail

Lethality in mammalian cells due to hyperthermia under oxic and hypoxic conditions.
MedLine Citation:
PMID:  304850     Owner:  NLM     Status:  MEDLINE    
From several studies of hyperthermia there have been reports that hypoxic cells are more sensitive to heat than their oxic counterparts. Experimental techniques in this investigation eliminate the effect of pH, trypsinization and cell attachment, when assaying the effects of hyperthermia on cells. Under hypoxic conditions, HeLa S3 and Chinese hamster cell-lines do not have an increased sensitivity to heat compared with oxic cells. HeLa S3 cells are protected against heat by hypoxia. Light-microscopy indicates the rupture of the plasma membrane, occasional nuclear budding, membrane vesicles and granulation of cell contents after heating at 43 degrees C for 3 hours. Scanning electron micrographs show that cells are more rounded after heat treatment and that there is an accompanying decrease in the number of microvilli, suggesting that the mechanism of cell attachment is affected. Heated cells should be delicately handled and subjected to the minimal trauma so that an accurate comparison of survival can be made.
H Bass; J L Moore; W T Coakley
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of radiation biology and related studies in physics, chemistry, and medicine     Volume:  33     ISSN:  0020-7616     ISO Abbreviation:  Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med.     Publication Date:  1978 Jan 
Date Detail:
Created Date:  1978-04-17     Completed Date:  1978-04-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0374725     Medline TA:  Int J Radiat Biol Relat Stud Phys Chem Med     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  57-67     Citation Subset:  IM    
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MeSH Terms
Cell Survival*
Hot Temperature*
Microscopy, Electron, Scanning
Reg. No./Substance:

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