Document Detail

Lethal effect of rifampicin in Bacillus subtilis as a complicating factor in the assessment of the lifetime of messenger ribonucleic acid.
MedLine Citation:
PMID:  4198759     Owner:  NLM     Status:  MEDLINE    
The bactericidal action of rifampicin was compared with that of chloramphenicol in growing and in sporulating cultures of Bacillus subtilis 168. Chloramphenicol kills cells only very slowly, but exposure to rifampicin kills over 95% of cells in a few minutes, causing gross physical damage, which is visible in both phase-contrast and electron microscopy. This is accompanied by a fall in O(2) consumption and by lysis. Experiments with synchronized cultures showed that susceptibility to the lethal effect of rifampicin is greater when the cells are dividing. The results suggest that the synthesis of some species of RNA other than mRNA may be necessary for the maintenance of cell integrity, although experiments with actinomycin D do not altogether fit this interpretation. However, we conclude that rifampicin is too toxic to use as an antibiotic for assessing the lifetime of mRNA.
J G Coote; D A Wood; J Mandelstam
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  134     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1973 May 
Date Detail:
Created Date:  1973-10-10     Completed Date:  1973-10-10     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  263-70     Citation Subset:  IM    
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MeSH Terms
Bacillus subtilis / cytology,  drug effects,  metabolism*
Bacterial Proteins / biosynthesis
Cell Count
Cell Division / drug effects
Chloramphenicol / pharmacology
Dactinomycin / pharmacology
Microscopy, Electron
Oxygen Consumption / drug effects
RNA, Bacterial / biosynthesis*
RNA, Messenger / biosynthesis*
Rifampin / pharmacology*
Spores, Bacterial / drug effects
Reg. No./Substance:
0/Bacterial Proteins; 0/RNA, Bacterial; 0/RNA, Messenger; 13292-46-1/Rifampin; 50-76-0/Dactinomycin; 56-75-7/Chloramphenicol

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