Document Detail


Lessons learned from the non-obese diabetic mouse II: Amelioration of pancreatic autoimmune isograft rejection during pregnancy.
MedLine Citation:
PMID:  8724729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine whether pregnancy provides an improved milieu for fetal/neonatal pancreas/islet transplantation, we studied neonatal pancreatic implants into non-obese diabetic (NOD) female mice during early gestation. We monitored maternal glycemic status, birthweight of the offspring, and graft histology to assess the efficacy of transplantation. One hundred and thirteen twelve-week-old NOD female mice were randomized into four groups as follows: (1) non-pregnant NOD mice received a sham operation; (2) non-pregnant NOD mice received neonatal pancreatic transplants; (3) pregnant NOD mice received a sham operation; and (4) pregnant NOD mice received neonatal pancreatic transplants. Pancreas segments from 3 neonatal NOD mice were placed via an incision 1 to 2 mm distal to the ear-skull junction of each of the recipients. Maternal blood glucose and glycated hemoglobin were determined between days 18 and 20 after the surgery. Pups were weighed within 5 to 6 hours after delivery. Pregnant NOD that received transplants (n = 29) had lower glucose and glycated hemoglobin (GHb) than sham operated pregnant controls (n = 26) (4.9 +/- 0.05 versus 9.0 +/- 5.0 mmol/L, p < 0.001 for glucose and 2.0 > or = 0.2 versus 3.0 > or = 1.2%, p < 0.008 for GHb) at 18 to 20 days of gestation. Controlling for litter size showed a decrease in birthweight for offspring of transplant recipients versus offspring of pregnant controls (1.59 +/- 0.08 versus 1.65 +/- 0.08 g, p < 0.002). Histological scoring of transplanted tissue at day 21 indicated that the lymphocytic infiltration in the pregnant group was significantly less than the control group (2.9 +/- 1.2 versus 4.9 +/- 0.2, p < 0.0001). We conclude that the pregnant NOD mouse provides a useful transplant model, that pregnancy provides an opportunity to increase beta-cell mass with transplanted tissue, and that pancreatic transplantation decrease birthweight and macrosomia in the offspring of NOD mice.
Authors:
H M Chen; L Jovanovic-Peterson; T A Desai; C M Peterson
Related Documents :
25013679 - Reducing stress and anxiety in caregivers of lung transplant patients: benefits of mind...
2675179 - Pancreatic transplant rejection: assessment with duplex us.
17175249 - Organogenesis of pancreatic anlagen allografted in rats.
18837609 - The role of interpersonal harm in distinguishing regret from guilt.
6259689 - Visibility and thickening of the renal fascia on computed tomograms.
2183429 - Evaluation of pancreatic allograft circulation using color doppler ultrasonography.
10037059 - Nephrotic syndrome as a clinical manifestation of graft-versus-host disease (gvhd) in a...
11360119 - Detection of ebv dna in the cord blood donor for a patient developing epstein-barr viru...
6382609 - Prolonged survival and remyelination after hematopoietic cell transplantation in the tw...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of perinatology     Volume:  13     ISSN:  0735-1631     ISO Abbreviation:  Am J Perinatol     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-10-16     Completed Date:  1996-10-16     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8405212     Medline TA:  Am J Perinatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  249-54     Citation Subset:  IM    
Affiliation:
Sansum Medical Research Foundation, Santa Barbara, California 93105, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Autoimmunity*
Blood Glucose / analysis
Female
Fetal Macrosomia
Graft Rejection / immunology*
Hemoglobin A, Glycosylated / analysis
Mice
Mice, Inbred NOD
Pancreas Transplantation / immunology*
Pregnancy
Pregnancy in Diabetics / blood,  immunology*,  therapy
Transplantation, Heterotopic
Transplantation, Isogeneic
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Hemoglobin A, Glycosylated

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Right diaphragmatic eventration simulating a congenital diaphragmatic hernia.
Next Document:  Three-dimensional reconstruction of vascular trees: experimental evaluation.