| Leptin and ObRa/MEK signalling in mouse oocyte maturation and preimplantation embryo development. | |
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MedLine Citation:
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PMID: 19712552 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent studies indicate that LH stimulates production of ovarian paracrine factors that induce meiosis of the oocyte. DNA microarray analyses of ovarian transcripts were performed in mice and major increases of a short isoform of leptin receptor, ObRa, were identified by the preovulatory LH/human chorionic gonadotrophin (HCG) surge. In oocytes, the level of ObRa transcripts was increased shortly after HCG stimulation, whereas the level of ObRb transcripts was not changed. Leptin was produced by cumulus, granulosa, theca and interstitial cells of ovaries and its transcript level was not regulated during gonadotrophin treatment. Treatment with leptin promoted germinal vesicle breakdown (GVBD) in oocytes within preovulatory follicles, and enhance first polar body extrusion in both cumulus-oocyte complexes and denuded oocytes. The leptin-promoted GVBD and first polar body extrusion were blocked by a mitogen-activated protein kinase extracellular signal regulated kinase kinases (MEK)1/2 inhibitor, U0126, but not its inactive analogue U0124. Furthermore, leptin promoted fertilization of oocytes and the in-vitro development of zygotes to preimplantation embryos. These findings suggest paracrine roles of leptin in the enhancement of nuclear maturation of oocytes through MEK1/2 signalling, and in the promotion of cytoplasmic maturation essential for successful oocyte development to the preimplantation embryos. |
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Authors:
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Yinghui Ye; Kazuhiro Kawamura; Mitsue Sasaki; Nanami Kawamura; Peter Groenen; Maarten D Sollewijn Gelpke; Jin Kumagai; Jun Fukuda; Toshinobu Tanaka |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Reproductive biomedicine online Volume: 19 ISSN: 1472-6491 ISO Abbreviation: Reprod. Biomed. Online Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-08-28 Completed Date: 2009-11-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101122473 Medline TA: Reprod Biomed Online Country: England |
Other Details:
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Languages: eng Pagination: 181-90 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Akita University School of Medicine, Akita, Japan. yeyh1999@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Blastocyst* Chorionic Gonadotropin / blood DNA Primers Female Immunohistochemistry Leptin / metabolism* Luteinizing Hormone / blood Mice Mice, Inbred ICR Oligonucleotide Array Sequence Analysis Oocytes / cytology* Receptors, Leptin / metabolism* Reverse Transcriptase Polymerase Chain Reaction Signal Transduction* |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 0/DNA Primers; 0/Leptin; 0/Receptors, Leptin; 9002-67-9/Luteinizing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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