Document Detail


Leptin-like immunoreactivity in the muscle of juvenile sea bass (Dicentrarchus labrax).
MedLine Citation:
PMID:  20146346     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mammalian hormone, leptin, is mainly synthesized in adipose tissue along with other tissues. Leptin plays a role in numerous processes such as in the control of food intake, homeostasis, immune function and reproduction. In this study, we detected and localized leptin immunoreactivity to the muscle of early juvenile sea bass (Dicentrarchus labrax) by Western blot analysis and immunohistochemistry. A leptin immunopositive band with a molecular weight of approximately 16 kDa, corresponding to mammalian leptin, was identified in trunk skeletal muscle homogenate. Furthermore, leptin immunopositive cells were detected in the endomysium of skeletal muscular fibers. These cells showed immunostained cytoplasmic granules and roundish and oval nuclei. The most intense immunostaining was observed in the endomysial space among the superficial red muscular fibers of the trunk. These findings suggest that in early juvenile sea bass, leptin is mostly produced by skeletal muscles. Therefore, during the developmental stage lacking adipose tissue, skeletal muscles can be considered an important source of leptin. As already suggested in mammals, we can hypothesize the potential roles of leptin not only in energy expenditure for muscle contraction but also during muscle differentiation and growth.
Authors:
Chiara Gambardella; Sara Ferrando; Lorenzo Gallus; Silvia Ravera; Paolo Bianchini; Paola Ramoino; Salvatore Fasulo; Grazia Tagliafierro
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microscopy research and technique     Volume:  73     ISSN:  1097-0029     ISO Abbreviation:  Microsc. Res. Tech.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  797-802     Citation Subset:  IM    
Copyright Information:
(c) 2010 Wiley-Liss, Inc.
Affiliation:
Department of Animal Biology and Marine Ecology, University of Messina, Messina 98166, Italy. chiara.gambardella@unige.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Bass*
Blotting, Western
Immunohistochemistry
Leptin / metabolism*
Muscle, Skeletal / chemistry*,  growth & development
Chemical
Reg. No./Substance:
0/Leptin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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