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Leptin and leucine synergistically regulate protein metabolism in C2C12 myotubes and mouse skeletal muscles.
MedLine Citation:
PMID:  23211060     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Leucine and leptin play important roles in regulating protein synthesis and degradation in skeletal muscles in vitro and in vivo. However, the objective of the present study was to determine whether leptin and leucine function synergistically in regulating protein metabolism of skeletal muscles. In the in vitro experiment, C2C12 myotubes were cultured for 2 h in the presence of 5 mm-leucine and/or 50 ng/ml of leptin. In the in vivo experiment, C57BL/6 and ob/ob mice were randomly assigned to be fed a non-purified diet supplemented with 3 % l-leucine or 2·04 % l-alanine (isonitrogenous control) for 14 d. Ob/ob mice were injected intraperitoneally with sterile PBS or recombinant mouse leptin (0·1 μg/g body weight) for 14 d. In C57BL/6 mice, dietary leucine supplementation increased (P < 0·05) plasma leptin, leptin receptor expression and protein synthesis in skeletal muscles, but reduced (P < 0·05) plasma urea and protein degradation in skeletal muscles. Dietary leucine supplementation and leptin injection increased the relative weight of the gastrocnemius and soleus muscles in ob/ob mice. Moreover, leucine and leptin treatments stimulated (P < 0·05) protein synthesis and inhibited (P < 0·05) protein degradation in C2C12 myotubes and skeletal muscles of ob/ob mice. There were interactions (P < 0·05) between the leucine and leptin treatments with regard to protein metabolism in C2C12 myotubes and soleus muscles of ob/ob mice but not in the gastrocnemius muscles of ob/ob mice. Collectively, these results suggest that leptin and leucine synergistically regulate protein metabolism in skeletal muscles both in vitro and in vivo.
Authors:
Xiangbing Mao; Xiangfang Zeng; Zhimin Huang; Junjun Wang; Shiyan Qiao
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-5
Journal Detail:
Title:  The British journal of nutrition     Volume:  -     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-9     Citation Subset:  -    
Affiliation:
State Key Laboratory on Animal Nutrition, China Agricultural University, No. 2, Yuanmingyuan West Road, Beijing 100193, People's Republic of China.
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