Document Detail


Leptin intake during the suckling period improves the metabolic response of adipose tissue to a high-fat diet.
MedLine Citation:
PMID:  20157325     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The intake of leptin during the suckling period protects against obesity and improves insulin and central leptin sensitivity in adult rats. OBJECTIVE: We analyzed whether leptin treatment to neonates may also improve later peripheral leptin sensitivity in adipose tissue under high-fat (HF) diet conditions. DESIGN: Male rats were supplemented with a daily oral dose of leptin or the vehicle (controls) during the suckling period. After weaning, animals were fed a normal-fat or an HF diet until the age of 6 months. At this age, mRNA and protein levels of the long-form leptin receptor (OB-Rb) and the expression of other genes related with energy metabolism were measured in various adipose depots (inguinal, mesenteric and retroperitoneal). RESULTS: HF-diet feeding resulted in lower OB-Rb mRNA and protein levels in internal depots in controls but not in leptin-treated animals; these animals maintained OB-Rb mRNA and protein levels under HF-diet conditions in these depots, particularly in the mesenteric one, and this was accompanied by increased expression of genes related with energy uptake (GLUT4, CD36), fatty acid oxidation (peroxisome proliferator activated receptor-alpha (PPARalpha), CPT1, UCP3) and lipogenesis (PPARgamma, GPAT). Leptin-treatment also ameliorated HF-diet-induced hepatic fat accumulation occurring in control animals. CONCLUSION: Leptin treatment during the suckling period may improve the lasting effects of HF-diet feeding on leptin receptor abundance in the adipose tissue and increase its oxidative capacity, resulting in a better handling and partitioning of excess fuel. This, together with the described improvement of central leptin sensitivity, may explain why these animals are more protected against diet-induced obesity and its metabolic-related disorders.
Authors:
T Priego; J Sánchez; A Palou; C Picó
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-16
Journal Detail:
Title:  International journal of obesity (2005)     Volume:  34     ISSN:  1476-5497     ISO Abbreviation:  Int J Obes (Lond)     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-10     Completed Date:  2010-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101256108     Medline TA:  Int J Obes (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  809-19     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Biology, University of the Balearic Islands, Palma de Mallorca, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / drug effects,  metabolism*
Animals
Animals, Suckling
Antigens, CD36 / metabolism
Blotting, Western
Body Weight / physiology
Dietary Fats / administration & dosage,  metabolism*
Energy Metabolism / physiology
Fatty Acids / metabolism
Gene Expression Regulation, Developmental / physiology
Glucose Transporter Type 4 / metabolism
Ion Channels / metabolism
Leptin / administration & dosage*,  metabolism
Male
Mitochondrial Proteins / metabolism
PPAR alpha / metabolism
PPAR gamma / metabolism
RNA, Messenger / metabolism
Rats
Rats, Wistar
Receptors, Leptin / metabolism*
Weaning
Chemical
Reg. No./Substance:
0/Antigens, CD36; 0/Dietary Fats; 0/Fatty Acids; 0/Glucose Transporter Type 4; 0/Ion Channels; 0/Leptin; 0/Mitochondrial Proteins; 0/PPAR alpha; 0/PPAR gamma; 0/RNA, Messenger; 0/Receptors, Leptin; 0/Slc2a4 protein, rat; 0/mitochondrial uncoupling protein 3
Comments/Corrections
Comment In:
Nat Rev Gastroenterol Hepatol. 2010 Jul;7(7):359   [PMID:  20626075 ]

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