| Leptin gene (TTTC)(n) microsatellite polymorphism in pre-eclampsia and HELLP syndrome. | |
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MedLine Citation:
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PMID: 19634986 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Leptin plays an important role in energy homeostasis. There is polymorphism on the leptin (LEP) gene. Our aim was to compare the tetranucleotide repeat (TTTC)(n) polymorphism in the 3'-flanking region in the LEP gene on DNA samples from patients with pre-eclampsia (PE), hemolysis, elevated liver enzymes, and low platelet (HELLP) syndrome and healthy pregnant controls. METHODS: Blood samples were collected from healthy pregnant women (n=88), patients with PE (n=79) and HELLP (n=77) syndrome. Fluorescent PCR and DNA fragment analysis was performed from the isolated DNA for the detection of (TTTC) repeats. The electrophoretograms were evaluated and patients were assigned to two groups; class I low (<190 bp) or class II high (> or =190 bp) PCR fragments. RESULTS: We observed similar distributions of the class I and class II (TTTC) alleles in the groups studied (class I allele: healthy pregnant 58.5%; severe pre-eclamptic 58.3%; HELLP syndrome 52.6%). We detected a higher frequency of the II/II genotype in HELLP syndrome patients (32.4%) compared to healthy controls (22.7%). However, the difference was not statistically significant. CONCLUSIONS: In an ethnically homogenous population, the LEP gene (TTTC) microsatellite polymorphism in the 3'-flanking region does not show a significant difference in the allele and genotype distribution in healthy pregnant, pre-eclamptic and HELLP syndrome patients. Furthermore, we recommend a new classification of the class I and class II alleles based on the distribution of the (TTTC) microsatellites. |
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Authors:
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Balint Nagy; Tibor Varkonyi; Attila Molvarec; Levente Lazar; Petronella Hupuczi; Nandor Gabor Than; Janos Rigo |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 47 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-09-04 Completed Date: 2009-11-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 1033-7 Citation Subset: IM |
Affiliation:
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First Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary. nabal@noi1.sote.hu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3' Flanking Region Adult Alleles Female Genotype HELLP Syndrome / genetics* Humans Leptin / genetics* Microsatellite Repeats / genetics* Polymorphism, Genetic Pre-Eclampsia / genetics* Pregnancy |
| Chemical | |
Reg. No./Substance:
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0/Leptin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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