| Leprecan distribution in the developing and adult kidney. | |
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MedLine Citation:
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PMID: 17495866 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The temporal and spatial deposition of extracellular matrix proteins is critical for nephrogenesis and glomerular maturation. We previously characterized leprecan as a novel chondroitin sulfate proteoglycan which has been recently shown to have prolyl hydroxylase activity. In this study, we examine the distribution of leprecan during nephrogenesis and after a hypertrophic stimulus to the adult kidney. During development, leprecan was localized to mesenchymal aggregates, early comma- and S-phase structures as determined by immunohistochemistry and in situ hybridization. Leprecan mRNA was increased in cells around the vascular cleft of the S- and comma-phase glomeruli. Expression was found in podocytes, mesangial cells, and parietal epithelial cells of loop-phase glomeruli. Leprecan mRNA was substantially decreased in the glomeruli of the adult kidney compared to the developing kidney with a uniform distribution between the glomeruli and the tubules. Within adult glomeruli, leprecan was found in the mesangium mesangial matrix, podocytes, and in Bowman's capsule. In response to glomerular hypertrophy, produced by unilateral nephrectomy, leprecan synthesis was increased in the adult kidney. We suggest that the regulated expression of leprecan during glomerular development or hypertrophy coupled with its reported prolyl hydroxylase activity plays a role during basement membrane assembly. |
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Authors:
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M Lauer; B Scruggs; S Chen; D Wassenhove-McCarthy; K J McCarthy |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-05-02 |
Journal Detail:
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Title: Kidney international Volume: 72 ISSN: 0085-2538 ISO Abbreviation: Kidney Int. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-06-28 Completed Date: 2007-09-06 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 0323470 Medline TA: Kidney Int Country: United States |
Other Details:
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Languages: eng Pagination: 82-91 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Cleveland Clinic, Cleveland, Ohio, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Bowman Capsule / growth & development, metabolism, pathology Cells, Cultured Disease Models, Animal Glomerular Mesangium / growth & development, metabolism, pathology Hypertrophy Kidney / growth & development*, metabolism*, pathology Kidney Glomerulus / growth & development, metabolism, pathology Male Membrane Glycoproteins / analysis, genetics, metabolism* Molecular Sequence Data Podocytes / metabolism, pathology Procollagen-Proline Dioxygenase / physiology Proteoglycans / analysis, genetics, metabolism* RNA, Messenger / genetics, metabolism Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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1-R01 DK48055/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Lepre1 protein, rat; 0/Membrane Glycoproteins; 0/Proteoglycans; 0/RNA, Messenger; EC 1.14.11.2/Procollagen-Proline Dioxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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