Document Detail

Leprecan distribution in the developing and adult kidney.
MedLine Citation:
PMID:  17495866     Owner:  NLM     Status:  MEDLINE    
The temporal and spatial deposition of extracellular matrix proteins is critical for nephrogenesis and glomerular maturation. We previously characterized leprecan as a novel chondroitin sulfate proteoglycan which has been recently shown to have prolyl hydroxylase activity. In this study, we examine the distribution of leprecan during nephrogenesis and after a hypertrophic stimulus to the adult kidney. During development, leprecan was localized to mesenchymal aggregates, early comma- and S-phase structures as determined by immunohistochemistry and in situ hybridization. Leprecan mRNA was increased in cells around the vascular cleft of the S- and comma-phase glomeruli. Expression was found in podocytes, mesangial cells, and parietal epithelial cells of loop-phase glomeruli. Leprecan mRNA was substantially decreased in the glomeruli of the adult kidney compared to the developing kidney with a uniform distribution between the glomeruli and the tubules. Within adult glomeruli, leprecan was found in the mesangium mesangial matrix, podocytes, and in Bowman's capsule. In response to glomerular hypertrophy, produced by unilateral nephrectomy, leprecan synthesis was increased in the adult kidney. We suggest that the regulated expression of leprecan during glomerular development or hypertrophy coupled with its reported prolyl hydroxylase activity plays a role during basement membrane assembly.
M Lauer; B Scruggs; S Chen; D Wassenhove-McCarthy; K J McCarthy
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-05-02
Journal Detail:
Title:  Kidney international     Volume:  72     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-28     Completed Date:  2007-09-06     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  82-91     Citation Subset:  IM    
Department of Biomedical Engineering, Cleveland Clinic, Cleveland, Ohio, USA.
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MeSH Terms
Amino Acid Sequence
Bowman Capsule / growth & development,  metabolism,  pathology
Cells, Cultured
Disease Models, Animal
Glomerular Mesangium / growth & development,  metabolism,  pathology
Kidney / growth & development*,  metabolism*,  pathology
Kidney Glomerulus / growth & development,  metabolism,  pathology
Membrane Glycoproteins / analysis,  genetics,  metabolism*
Molecular Sequence Data
Podocytes / metabolism,  pathology
Procollagen-Proline Dioxygenase / physiology
Proteoglycans / analysis,  genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
Rats, Sprague-Dawley
Grant Support
Reg. No./Substance:
0/Lepre1 protein, rat; 0/Membrane Glycoproteins; 0/Proteoglycans; 0/RNA, Messenger; EC Dioxygenase

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