Document Detail

Lentivirus as a tool for lineage-specific gene manipulations.
MedLine Citation:
PMID:  17607693     Owner:  NLM     Status:  MEDLINE    
The trophectoderm (TE) of blastocysts, the first epithelium established in mammalian development, (1) plays signaling, supportive, and patterning functions during preimplantation development, (2) ensures embryo implantation into the uterine wall, and (3) gives rise to extraembryonic tissues essential for embryo patterning and growth after implantation. We show that mouse TE, itself permissive to lentiviral (LV) infection, represents a robust nonpermeable physical barrier to the virus particles, thereby shielding the cells of the inner cell mass from viral infection. This LV feature will allow modulations of gene expression in a lineage-specific manner, thus having significant applications in mouse functional genetics.
Anna Malashicheva; Benoît Kanzler; Elena Tolkunova; Didier Trono; Alexey Tomilin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  45     ISSN:  1526-954X     ISO Abbreviation:  Genesis     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-12     Completed Date:  2007-09-19     Revised Date:  2008-04-04    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  456-9     Citation Subset:  IM    
Department of Developmental Biology, Max-Planck Institute for Immunobiology, Freiburg, Germany.
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MeSH Terms
Cell Lineage*
Gene Transfer Techniques*
Genes, Reporter
Genetic Vectors*
Mice, Inbred C57BL
Erratum In:
Genesis. 2007 Dec;45(12):793

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