| Lentiviral vector-mediated knockdown of SOCS3 in the hypothalamus protects against the development of diet-induced obesity in rats. | |
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MedLine Citation:
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PMID: 21554522 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Aim: Leptin resistance is a feature of most cases of obesity in both humans and rodents. The suppressor of cytokine signaling 3 (SOCS3) is a negative-feedback regulator of leptin signaling involved in leptin resistance, therefore the suppression of SOCS3 is a potential therapy for leptin-resistance in obesity. In the studies, we investigated whether hypothalamic silencing of SOCS3 would attenuate diet-induced obesity in rats. Methods: First we established hypothalamic SOCS3-deficient rats through lentiviral vector mediated RNA interference technique, then provided a high-fat diet or a chow diet to the rats. After 8 weeks of the diet, the serum leptin and insulin concentrations were measured by RIA, and the gene expressions of SOCS3 and the long form of the leptin receptor in hypothalamus were detected by a real time RT-PCR. The Leptin-induced Stat3 activation was examined by Western blot. Results: The RNAi protocol specifically knocked down the expression of SOCS3 mRNA by 50% approximately. The rats treated with LV-SOCS3-shRNA exhibited enhanced leptin-induced Stat3 activation, decreased body weight gain and improved metabolic parameters when exposed to a high-fat diet. Conclusion: Our results provide evidence that the rats treated with hypothalamic SOCS3 silencing are significantly protected against the development of diet-induced obesity and SOCS3 is a potential target molecule for therapeutic intervention of obesity. |
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Authors:
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Zheng-Juan Liu; Jie Bian; Yong-Li Zhao; Xu Zhang; Ning Zou; Dan Li |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-6 |
Journal Detail:
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Title: Diabetes, obesity & metabolism Volume: - ISSN: 1463-1326 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883645 Medline TA: Diabetes Obes Metab Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 Blackwell Publishing Ltd. |
Affiliation:
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Department of Pediatrics, the Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, Shahekou District, Dalian 116027, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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