| Leishmania mexicana lipophosphoglycan differentially regulates PKCalpha-induced oxidative burst in macrophages of BALB/c and C57BL/6 mice. | |
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MedLine Citation:
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PMID: 20500675 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Leishmania are protozoan parasites that infect macrophages and their survival is partially achieved through inhibition of the cellular oxidative burst by parasite lipophosphoglycan (LPG). PKCalpha is the predominant PKC isoenzyme required for macrophage oxidative burst, yet it is not known if different susceptibility of BALB/c and C57BL/6 mice to Leishmania mexicana could be related to PKCalpha. We analysed the effect of L. mexicana promastigotes and parasite LPG on expression of PKCalpha and on its activity in macrophages of both mouse strains. Our data show that expression of the isoenzyme was not altered either by LPG or by L. mexicana promastigotes. Yet LPG exerted opposing effects on PKCalpha activity of macrophages between both strains: in susceptible BALB/c cells, it inhibited PKCalpha activity, whereas in the more resistant strain it augmented enzymatic activity 2.8 times. In addition, LPG inhibited oxidative burst only in susceptible BALB/c macrophages and the degree of inhibition correlated with parasite survival. Promastigotes also inhibited PKCalpha activity and oxidative burst in macrophages of BALB/c mice, whereas in C57BL/6, they enhanced PKCalpha activity and oxidative burst inhibition was less severe. Our data indicate that control of PKCalpha-induced oxidative burst by L. mexicana LPG relates with its success to infect murine macrophages. |
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Authors:
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J Delgado-Domínguez; H González-Aguilar; M Aguirre-García; L Gutiérrez-Kobeh; M Berzunza-Cruz; A Ruiz-Remigio; M Robles-Flores; I Becker |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Parasite immunology Volume: 32 ISSN: 1365-3024 ISO Abbreviation: Parasite Immunol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-26 Completed Date: 2010-08-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7910948 Medline TA: Parasite Immunol Country: England |
Other Details:
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Languages: eng Pagination: 440-9 Citation Subset: IM |
Affiliation:
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Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Colonia Doctores, México D.F., México. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Disease Susceptibility / immunology Gene Expression Profiling Glycosphingolipids / metabolism* Leishmania mexicana / pathogenicity* Leishmaniasis, Diffuse Cutaneous / immunology, parasitology Macrophages / immunology*, parasitology* Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Protein Kinase C-alpha / antagonists & inhibitors*, biosynthesis* Respiratory Burst* Survival Analysis |
| Chemical | |
Reg. No./Substance:
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0/Glycosphingolipids; 0/lipophosphonoglycan; EC 2.7.11.13/Protein Kinase C-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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