| Leishmania mexicana: inhibition of camptothecin-induced apoptosis of monocyte-derived dendritic cells. | |
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MedLine Citation:
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PMID: 19041644 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Macrophages (Mphi) and dendritic cells (DC) are the major target cell populations of the obligate intracellular parasite Leishmania. Inhibition of host cell apoptosis is a method employed by multiple pathogens to ensure their survival in the infected cell. Leishmania has been shown to protect Mphi and neutrophils from both natural and induced apoptosis. As shown in this study, apoptosis in monocyte-derived dendritic cells (moDC) induced by treatment with camptothecin was downregulated by coincubation with L. mexicana, as detected by morphological analysis of cell nuclei, TUNEL assay, gel electrophoresis of low molecular weight DNA fragments, and annexin V binding to phosphatidylserine. The observed antiapoptotic effect was found to be associated with a significant reduction of caspase-3 activity in moDC. The capacity of L. mexicana to delay apoptosis induction in the infected moDC may have implications for Leishmania pathogenesis by favoring the invasion of its host and the persistence of the parasite in the infected cells. |
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Authors:
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Leonardo Valdés-Reyes; Jesús Argueta; Julio Morán; Norma Salaiza; Joselín Hernández; Miriam Berzunza; Magdalena Aguirre-García; Ingeborg Becker; Laila Gutiérrez-Kobeh |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-11-18 |
Journal Detail:
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Title: Experimental parasitology Volume: 121 ISSN: 1090-2449 ISO Abbreviation: Exp. Parasitol. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-02-23 Completed Date: 2009-03-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: United States |
Other Details:
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Languages: eng Pagination: 199-207 Citation Subset: IM |
Affiliation:
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Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Dr. Balmis 148, Col. Doctores, México, D.F. 06726, Mexico. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents, Phytogenic / pharmacology* Apoptosis / drug effects, physiology* Azure Stains Camptothecin / pharmacology* Caspase 3 / metabolism Cell Survival Cells, Cultured DNA Fragmentation Dendritic Cells / cytology, drug effects, parasitology* Down-Regulation Flow Cytometry Humans In Situ Nick-End Labeling Leishmania mexicana / physiology* Monocytes / cytology |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/Azure Stains; 7689-03-4/Camptothecin; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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