| Leishmania major: Reactive oxygen species and interferon gamma induction by soluble lipophosphoglycan of stationary phase promastigotes. | |
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MedLine Citation:
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PMID: 16759653 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Protozoan parasites of the genus Leishmania cause a number of important human diseases. One of the key determinants of parasite infectivity and survival is membrane glycoconjugate lipophosphoglycan (mLPG). In addition, it has been shown that mLPG could be used as a transmission blocking vaccine. Since culture supernatant of parasite promastigotes is a good source of LPG, we attempted to compare the immunological properties of culture supernatant and membrane LPG prepared from stationary phase promastigotes of Leishmania major. The purity of supernatant LPG (sLPG) and membrane LPG (mLPG) was determined by thin layer chromatography. The effect of sLPG and mLPG on the production of reactive oxygen species (ROS) was studied using PBMCs isolated from healthy individuals. In addition, induction of IL-12, IFN-gamma and IL-10 secretion in the presence of sLPG and mLPG was investigated. Reactive oxygen species in addition to IL-10 and IL-12 were induced by both sLPG and mLPG. However, IFN-gamma production was promoted only in response to sLPG suggesting its ability to promote Th1 response and implication in vaccine design. |
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Authors:
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Gholamreza Kavoosi; Sussan K Ardestani; Amina Kariminia; Mohssen Abolhassani; Salvatore J Turco |
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Publication Detail:
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Type: Journal Article Date: 2006-06-08 |
Journal Detail:
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Title: Experimental parasitology Volume: 114 ISSN: 0014-4894 ISO Abbreviation: Exp. Parasitol. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-10-30 Completed Date: 2007-01-09 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: United States |
Other Details:
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Languages: eng Pagination: 323-8 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry and Biophysics, University of Tehran, P.O.Box 13148-1384, Tehran, Iran. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Cell Membrane / chemistry, immunology Chromatography, Thin Layer Glycosphingolipids / chemistry, immunology*, isolation & purification, pharmacology Humans Interferon-gamma / biosynthesis* Interleukin-10 / biosynthesis Interleukin-12 / biosynthesis Leishmania major / immunology* Leishmaniasis, Cutaneous / prevention & control Leukocytes, Mononuclear / drug effects, immunology*, metabolism Mice Mice, Inbred BALB C Protozoan Vaccines / chemistry Reactive Oxygen Species / metabolism* Solubility |
| Chemical | |
Reg. No./Substance:
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0/Glycosphingolipids; 0/Protozoan Vaccines; 0/Reactive Oxygen Species; 0/lipophosphonoglycan; 130068-27-8/Interleukin-10; 187348-17-0/Interleukin-12; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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