Document Detail


Leishmania braziliensis: characterisation of a complex specific subtelomeric repeat sequence and its use in the detection of parasites.
MedLine Citation:
PMID:  9806868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A 1.6-kb tandem repeat sequence had previously been identified in the subtelomeric region of mini- and megabase chromosomes from Leishmania braziliensis. Southern hybridisation was used to demonstrate that the repeat is complex specific. The sequence was characterised in strains representing four species of the L. braziliensis complex. This data allowed an assessment of the evolutionary relationship of the four species. PCR primers targeted to the repeat amplify only DNA from species of the L. braziliensis complex. Titration assays indicate that a minimum of 50 fg of parasite DNA can be detected by PCR alone. Southern hybridisation increases the limit of detection to 5 fg. Interspecies variation in the repeat sequence enabled restriction enzyme digestion of PCR products to distinguish individual species within the L. braziliensis complex.
Authors:
G Fu; G Perona-Wright; D C Barker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental parasitology     Volume:  90     ISSN:  0014-4894     ISO Abbreviation:  Exp. Parasitol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1998-12-01     Completed Date:  1998-12-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370713     Medline TA:  Exp Parasitol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  236-43     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
Affiliation:
MRC Outstation of NIMR, Molteno Laboratories, Department of Pathology, University of Cambridge, CB2 1QP, U.K.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF031224;  AF043685;  AF043686;  AF043687
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Blotting, Southern
Conserved Sequence
DNA Primers / chemistry
DNA, Protozoan / chemistry*
DNA, Ribosomal / chemistry
Leishmania braziliensis / classification,  genetics*,  isolation & purification
Leishmaniasis, Cutaneous / diagnosis*
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Restriction Mapping
Sequence Alignment
Species Specificity
Tandem Repeat Sequences / genetics*
Telomere / genetics*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Protozoan; 0/DNA, Ribosomal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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