| Leishmania braziliensis: characterisation of a complex specific subtelomeric repeat sequence and its use in the detection of parasites. | |
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MedLine Citation:
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PMID: 9806868 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A 1.6-kb tandem repeat sequence had previously been identified in the subtelomeric region of mini- and megabase chromosomes from Leishmania braziliensis. Southern hybridisation was used to demonstrate that the repeat is complex specific. The sequence was characterised in strains representing four species of the L. braziliensis complex. This data allowed an assessment of the evolutionary relationship of the four species. PCR primers targeted to the repeat amplify only DNA from species of the L. braziliensis complex. Titration assays indicate that a minimum of 50 fg of parasite DNA can be detected by PCR alone. Southern hybridisation increases the limit of detection to 5 fg. Interspecies variation in the repeat sequence enabled restriction enzyme digestion of PCR products to distinguish individual species within the L. braziliensis complex. |
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Authors:
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G Fu; G Perona-Wright; D C Barker |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental parasitology Volume: 90 ISSN: 0014-4894 ISO Abbreviation: Exp. Parasitol. Publication Date: 1998 Nov |
Date Detail:
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Created Date: 1998-12-01 Completed Date: 1998-12-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 236-43 Citation Subset: IM |
Copyright Information:
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Copyright 1998 Academic Press. |
Affiliation:
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MRC Outstation of NIMR, Molteno Laboratories, Department of Pathology, University of Cambridge, CB2 1QP, U.K. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF031224; AF043685; AF043686; AF043687 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Blotting, Southern Conserved Sequence DNA Primers / chemistry DNA, Protozoan / chemistry* DNA, Ribosomal / chemistry Leishmania braziliensis / classification, genetics*, isolation & purification Leishmaniasis, Cutaneous / diagnosis* Molecular Sequence Data Phylogeny Polymerase Chain Reaction Restriction Mapping Sequence Alignment Species Specificity Tandem Repeat Sequences / genetics* Telomere / genetics* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Protozoan; 0/DNA, Ribosomal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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