| Legionella pneumophila secretes an endoglucanase that belongs to the family-5 of glycosyl hydrolases and is dependent upon type II secretion. | |
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MedLine Citation:
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PMID: 19817866 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Examination of cell-free culture supernatants revealed that Legionella pneumophila strains secrete an endoglucanase activity. Legionella pneumophila lspF mutants were deficient for this activity, indicating that the endoglucanase is secreted by the bacterium's type II protein secretion (T2S) system. Inactivation of celA, encoding a member of the family-5 of glycosyl hydrolases, abolished the endoglucanase activity in L. pneumophila culture supernatants. The cloned celA gene conferred activity upon recombinant Escherichia coli. Thus, CelA is the major secreted endoglucanase of L. pneumophila. Mutants inactivated for celA grew normally in protozoa and macrophage, indicating that CelA is not required for the intracellular phase of L. pneumophila. The CelA endoglucanase is one of at least 25 proteins secreted by the type II system of L. pneumophila and the 17th type of enzyme effector associated with this pathway. Only a subset of the other Legionella species tested expressed secreted endoglucanase activity, suggesting that the T2S output differs among the different legionellae. Overall, this study represents the first documentation of an endoglucanase (EC 3.2.1.4) being produced by a strain of Legionella. |
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Authors:
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Meghan M Pearce; Nicholas P Cianciotto |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-09-21 |
Journal Detail:
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Title: FEMS microbiology letters Volume: 300 ISSN: 1574-6968 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-23 Completed Date: 2009-12-16 Revised Date: 2011-10-03 |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: England |
Other Details:
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Languages: eng Pagination: 256-64 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St., Chicago, IL 60611, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acanthamoeba castellanii
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microbiology Animals Cells, Cultured Cellulase / secretion* Cloning, Molecular Colony Count, Microbial Escherichia coli / genetics, metabolism Gene Knockout Techniques Hartmannella / microbiology Legionella pneumophila / enzymology* Macrophages / microbiology Mice Mice, Inbred A Virulence |
| Grant Support | |
ID/Acronym/Agency:
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AI43987/AI/NIAID NIH HHS; R01 AI043987-11/AI/NIAID NIH HHS; R01 AI043987-13/AI/NIAID NIH HHS; R01 AI043987-14/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.2.1.4/CelA endoglucanase; EC 3.2.1.4/Cellulase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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