Document Detail

Lefty is expressed in mouse endometrium in estrous cycle and peri-implantation period.
MedLine Citation:
PMID:  15640253     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Reproductive tissues are unique structures that exhibit cyclic stromal remodelling during menstrual cycles in humans. Ebaf/lefty participates in tissue remodelling of human endometrium by induction of matrix metalloproteases (MMP). METHODS: We describe the temporal expression and spatial distribution of lefty and tissue remodelling events in mouse endometrium. RT-PCR and real-time PCR were used to identify mRNA expression and western blots to analyse Lefty protein. Immunolocalization was performed with specific antibodies and horseradish peroxidase staining. RESULTS: Lefty was expressed in endometrium throughout the estrous cycle. Expression of MMP (MMP-2, -3, -7 and -14) was higher at estrus, metestrus and/or diestrus while collagen content of endometrium decreased in these phases. During pregnancy, lefty levels were higher on days 3-5 and were minimal by day 9. Similarly, expression of endometrial MMP was higher on days 3 and 5 of pregnancy and was low on day 9. During pregnancy, loss of collagen was initiated on day 3, persisted to day 5, and led to a significantly reduced collagen on day 9. Immunoreactive lefty decorated basal laminae, and was associated with extracellular matrix in stroma. CONCLUSIONS: Regulated expression and spatial distribution of lefty in mouse endometrium confines its biological impact on tissues that undergo remodelling during estrous cycle and pregnancy.
Meiyi Tang; Yun Xu; Joanne Julian; Daniel Carson; Siamak Tabibzadeh
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2005-01-07
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  20     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-24     Completed Date:  2005-06-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  872-80     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Stony Brook University, Stony Brook, NY, USA.
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MeSH Terms
Collagen / metabolism
Embryo Implantation / physiology*
Endometrium / physiology*
Estrous Cycle / physiology*
Gene Expression Regulation
Left-Right Determination Factors
Matrix Metalloproteinases / genetics
Mice, Inbred Strains
Pregnancy, Animal / physiology*
RNA, Messenger / analysis
Transforming Growth Factor beta / genetics*,  metabolism
Grant Support
Reg. No./Substance:
0/LEFTY1 protein, human; 0/Left-Right Determination Factors; 0/Lefty2 protein, mouse; 0/RNA, Messenger; 0/Transforming Growth Factor beta; 9007-34-5/Collagen; EC 3.4.24.-/Matrix Metalloproteinases

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