Document Detail


Left ventricular volumes and function in the embryonic mouse heart.
MedLine Citation:
PMID:  9321827     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study describes miniaturized technology for the in vivo analysis of the volume and function of the embryonic mouse heart and the application of this technology to study the normal embryonic left ventricle (LV) at two stages of development. With the use of microsurgical techniques, embryos from embryonic day (ED) 10.5 (ED10.5) to ED16 were delivered individually from litters of normal dams, and cardiac visualization was achieved with the use of intravital microscopy by transillumination, with the umbilical circulation intact. At ED10.5-11, the heart could be imaged in color in the intact embryo, whereas at ED12.5 it was necessary to open the chest; at ED13.5-14.5, fluorescent imaging with the use of microinjection of fluorescein-conjugated albumin was necessary to visualize the LV chamber. At ED10.5-11, the LV end-diastolic volumes averaged 0.16 microliter (n = 14), and at ED13.5-14.5, they averaged 0.57 microliter (n = 16). At both ages there was a positive linear relationship between the LV end-diastolic volume and the stroke volume despite substantial variations in individual heart rates, reflecting the relative uniformity of the LV ejection fractions within each age group. The average of the individual ejection fractions was 27.4% at ED10.5-11 and 58.4% at ED13.5-14.5, the latter being within the normal range for the adult rodent heart. These methods will be useful for assessing in vivo cardiac function at ED10.5 and older murine embryos in litters of transgenic or gene-targeted mice when the mutation leads to later embryonic lethality.
Authors:
N Tanaka; L Mao; F A DeLano; E M Sentianin; K R Chien; G W Schmid-Schönbein; J Ross
Related Documents :
20300087 - The effect of surgical weight reduction on left ventricular structure and function in s...
18657667 - A randomized trial of the aldosterone-receptor antagonist eplerenone in asymptomatic mo...
24030417 - Creation of nonischemic functional mitral regurgitation by annular dilatation and nonpl...
7982417 - Na,k-pump concentration in hypertrophied human hearts.
20131007 - Central role of real-time three-dimensional echocardiography in the assessment of intra...
16160907 - Chest stab wound-related coronary artery pseudoaneurysm sealed with a polytetrafluoroet...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  273     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-10-23     Completed Date:  1997-10-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1368-76     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of California San Diego, La Jolla 92093-0613, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Coronary Angiography
Diastole
Embryonic and Fetal Development
Female
Fetal Heart / anatomy & histology,  physiology*
Gestational Age
Heart Rate, Fetal
Heart Ventricles
Mice
Mice, Inbred Strains
Microscopy, Video
Pregnancy
Regression Analysis
Ventricular Function, Left*
Grant Support
ID/Acronym/Agency:
HL-46345/HL/NHLBI NIH HHS; HL-51549/HL/NHLBI NIH HHS; HL-55926/HL/NHLBI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Downregulation of cyclin-dependent kinase inhibitors p21 and p27 in pressure-overload hypertrophy.
Next Document:  Three-dimensional activation sequence of cesium-induced ventricular arrhythmias.