| Lectins from bulbs of the Chinese daffodil Narcissus tazetta (family Amaryllidaceae). | |
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MedLine Citation:
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PMID: 11012085 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The isolation of three lectins with similar N-terminal amino acid sequences from the bulbs of the Chinese daffodil Narcissus tazetta was achieved. The isolation protocol involved ion exchange chromatography on DEAE-cellulose, affinity chromatography on mannose-agarose, and fast protein liquid chromatography-gel filtration on Superose 12. The lectins were all adsorbed on mannose-agarose and demonstrated a single band with a molecular weight of 13 kDa in SDS-polyacrylamide gel electrophoresis and a single 26 kDa peak in gel filtration, indicating that they were mannose-binding, dimeric proteins. The lectins differed in hemagglutinating activity, with the magnitude of the activity correlating with the ionic strength of the buffer required to elute the lectin from the DEAE-cellulose column. The bulb lectin did not exert potent cytotoxicity against cancer cell lines or fetal bovine lung cells but inhibited syncytium formation in, and reinstated viability of, fetal bovine lung cells infected with bovine immunodeficiency virus. |
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Authors:
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L S Ooi; T B Ng; Y Geng; V E Ooi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry and cell biology = Biochimie et biologie cellulaire Volume: 78 ISSN: 0829-8211 ISO Abbreviation: Biochem. Cell Biol. Publication Date: 2000 |
Date Detail:
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Created Date: 2001-01-03 Completed Date: 2001-01-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8606068 Medline TA: Biochem Cell Biol Country: CANADA |
Other Details:
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Languages: eng Pagination: 463-8 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Angiosperms / chemistry* Animals Cattle Cell Death / drug effects Cell Line Cell Survival / drug effects Chromatography Cytopathogenic Effect, Viral / drug effects Drug Screening Assays, Antitumor Electrophoresis, Polyacrylamide Gel Giant Cells / drug effects Hemagglutination Tests Humans Immunodeficiency Virus, Bovine / drug effects, physiology Lectins / chemistry*, isolation & purification*, pharmacology Lung Mice Molecular Sequence Data Plant Lectins Plant Proteins / chemistry, isolation & purification, pharmacology Sequence Alignment Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Lectins; 0/Plant Lectins; 0/Plant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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