| Lectin-induced activation of plasma membrane NADPH oxidase in cholesterol-depleted human neutrophils. | |
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MedLine Citation:
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PMID: 22056482 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The gp91phox subunit of flavocytochrome b(558) is the catalytic core of the phagocyte plasma membrane NADPH oxidase. Its activation occurs within lipid rafts and requires translocation of four subunits to flavocytochrome b(558). gp91phox is the only glycosylated subunit of NADPH oxidase and no data exist about the structure or function of its glycans. Glycans, however, bind to lectins and this can stimulates NADPH oxidase activity. Given this information, we hypothesized that lectin-gp91phox interactions would facilitate the assembly of a functionally active NADPH oxidase in the absence of lipid rafts. To test this, we used lectins with different carbohydrate-binding specificity to examine the effects on H(2)O(2) generation by human neutrophils treated with the lipid raft disrupting agent methyl-β-cyclodextrin (MβCD). MβCD treatment removed membrane cholesterol, caused changes in cell morphology, inhibited lectin-induced cell aggregation, and delayed lectin-induced assembly of the NADPH oxidase complex. More importantly, MβCD treatment either stimulated or inhibited H(2)O(2) production in a lectin-dependent manner. Together, these results show selectivity in lectin binding to gp91phox, and provide evidence for the biochemical structures of the gp91phox glycans. Furthermore, the data also indicate that in the absence of lipid rafts, neutrophil NADPH oxidase activity can be altered by these select lectins. |
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Authors:
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Irina V Gorudko; Ann V Mukhortava; Brendan Caraher; Melody Ren; Sergey N Cherenkevich; Gregory M Kelly; Alexander V Timoshenko |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-28 |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: - ISSN: 1096-0384 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-11-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
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Department of Biophysics, Belarusian State University, Minsk 220030, Belarus. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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