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Lectin from Agaricus bisporus inhibited S phase cell population and Akt phosphorylation in human retinal pigment epithelial cells.
MedLine Citation:
PMID:  23036999     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Purpose. Lectin from the edible mushroom Agaricus bisporus (ABL) was found to inhibit cell proliferation of some ocular and cancer cell-lines. To elucidate how ABL inhibited retinal pigment epithelial (RPE) cell proliferation, we investigated the changes in cell cycle distribution and cell proliferation-related signalling pathways after ABL treatment. Methods. Primary human RPE cells were isolated and grown in DMEM/F12 with or without the ABL (20 or 90μg/ml) for three days. Analysis of cell cycle was performed by flow cytometry. Phosphorylation status of Erk, Jnk, p38, and Akt as well as p53 expression levels were investigated by Western blotting. The role of phosphorylated-Akt in RPE cell proliferation was further evaluated using LY294002. Results. After ABL treatment (90μg/ml), the amount of cells present in the S phase was found to be reduced. These changes were not apparent in cells treated with 20μg/ml ABL. In addition, Erk and Akt were found to be hyper-phosphorylated and hypo-phosphorylated, respectively. The expression levels of phosphorylated-Jnk, phosphorylated-p38 and p53 were not altered when compared with those of the control cells. When RPE cells were treated with LY294002 and deprived from phosphorylated-Akt expression, cell proliferation rate was reduced. Reduction in the amount of cells present in S phase was also observed. Conclusions. Our results showed that ABL hypo-phosphorylated Akt and this observation is in line with the fact that ABL attenuates cell proliferation. As the level of p53 was not significantly altered by ABL, this indicated that ABL-arrested cell cycle progression was independent of p53 activation.
Authors:
Yiu-Him Cheung; Carl M Sheridan; Amy C Y Lo; Wico W Lai
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  -     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Eye Institute, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong.
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