| Lectin electron microscopic histochemistry of the pseudoexfoliative material in the skin. | |
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MedLine Citation:
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PMID: 7928196 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To investigate the hypothesis that pseudoexfoliation (PSX) syndrome is a systemic disorder, the authors studied the composition of glycoconjugates in the intraocular and extraocular PSX material at the electron microscopic level, using a panel of lectins as cytochemical probes. METHODS: The authors examined 8 lid skins, 11 trabecular tissues, and 3 cataractous lenses from human eyes with PSX syndrome. Tissues were processed for electron microscopical histochemistry and stained with PNA, RCA120, DBA, SBA, ConA, WGA, UEA-I, and Lotus, with an indirect lectin-colloidal gold technique. RESULTS: Both the intraocular and extraocular PSX materials manifested almost identical reactivity to lectins, which indicated that glycoconjugates in the PSX material contained with sugar residues of galactose (PNA, RCA120), alpha-mannose (ConA), and N-acetyl-D-glucosamine (WGA). On the other hand, it was indicated that sugar residues of N-acetyl-D-galactosamine (DBA, SBA) and fucose (UEA-I, Lotus) were absent. Granular inclusions and microfibrils in the capsule and ocular zonules were stained similarly and weakly. CONCLUSIONS: The intraocular and extraocular PSX materials contained the same sugar residues of glycoconjugates, which suggested that those materials had the same nature. This study, the first documentation of lectin-binding sites on the extraocular PSX material, supported the hypothesis of PSX syndrome as a systemic disorder. |
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Authors:
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F Amari; S Nagata; J Umihira; M Nohara; N Usuda; K Segawa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 35 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 1994 Oct |
Date Detail:
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Created Date: 1994-11-15 Completed Date: 1994-11-15 Revised Date: 2010-03-24 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3962-6 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Exfoliation Syndrome
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metabolism*,
pathology Eyelids / metabolism, ultrastructure Glycoconjugates / metabolism* Histocytochemistry Humans Immunohistochemistry Lectins / metabolism Lens, Crystalline / metabolism, ultrastructure Microscopy, Electron Oligosaccharides / analysis Skin / metabolism*, ultrastructure Trabecular Meshwork / metabolism, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Glycoconjugates; 0/Lectins; 0/Oligosaccharides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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