Document Detail


Lectin-binding pattern as tool to identify and enrich specific primary testis cells of the tilapia (Oreochromis niloticus) and medaka (Oryzias latipes).
MedLine Citation:
PMID:  16788897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell type-specific lectin binding is a useful tool for the analysis of developing systems. We describe the binding pattern of 21 different fluorescein isothiocyanate (FITC)-labelled lectins to the testis of two model teleost species, the medaka (Oryzias latipes) and the tilapia (Oreochromis niloticus). The analysis of the binding pattern was carried out on tissue sections (medaka and tilapia) and using primary culture cells (only tilapia). Lectin binding was studied by confocal microscopy and for histological analysis some sections were, in addition, stained with bodipy to gain additional information concerning the cytological organization of the cystic mode of spermatogenesis in fish. The observed differences in lectin staining of different cell types in primary cultures were quantified by flow cytometry. Only few lectins bound specifically to haploid cells while the reaction to diploid or tetraploid cells was generally stronger. However, the extracellular material around the haploid spermatids and spermatozoa in spermatocysts showed a strong staining reaction with several lectins (e.g., Phaseolus vulgaris Erythro agglutinin). The apparent differences in the cellular lectin-binding pattern can be used to identify particular cell types, to monitor their differentiation in vitro or to enrich particular cell types from heterogeneous cultures using magnetic beads coated with anti-FITC antibodies. Using the latter approach, we show that it is possible to enrich for gonial cells and at the same time deplete the preparation for haploid cells and Sertoli cells.
Authors:
Sergey V Tokalov; Herwig O Gutzeit
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of experimental zoology. Part B, Molecular and developmental evolution     Volume:  308     ISSN:  1552-5007     ISO Abbreviation:  J. Exp. Zool. B Mol. Dev. Evol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-15     Completed Date:  2007-06-11     Revised Date:  2009-10-05    
Medline Journal Info:
Nlm Unique ID:  101168228     Medline TA:  J Exp Zool B Mol Dev Evol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  127-38     Citation Subset:  IM    
Copyright Information:
(c) 2006 Wiley-Liss, Inc.
Affiliation:
Institut für Zoologie, TU Dresden, D-01062 Dresden, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Boron Compounds
Cells, Cultured
Cichlids / metabolism*
Extracellular Fluid / metabolism
Flow Cytometry
Fluorescein-5-isothiocyanate
Histocytochemistry
Lectins / metabolism*
Male
Microscopy, Confocal
Oryzias / metabolism*
Protein Binding
Spermatogenesis / physiology*
Testis / cytology*,  metabolism
Chemical
Reg. No./Substance:
0/4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; 0/Boron Compounds; 0/Lectins; 3326-32-7/Fluorescein-5-isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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