Document Detail


Lectin binding in Cryptomonas and Chroomonas (Cryptophyceae).
MedLine Citation:
PMID:  21927885     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The cell envelopes of Cryptomonas and Chroomonas exhibited significant fluorescence using FITC-labelled concanavalin A and wheat germ agglutinin when the cells were fixed prior to lectin binding. The periplast became intensely labelled in Chroomonas whereas Cryptomonas showed fluorescing granula in its gullet/furrow region and on the cell surface. Lectin labelling followed by fixation showed only label of periplast remnants of lysed cells and of the flagella of Chroomonas. Isolated periplasts of Cryptomonas and Chroomonas were intensively labelled with both concanavalin A and wheat germ agglutinin. Glycostaining of gels, onto which total cell protein extracts were loaded, showed a glycoprotein of high molecular weight for Cryptomonas and Chroomonas and an additional glycoprotein for Cryptomonas species.
Authors:
Erhard Rhiel; Jörg Brock
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-18
Journal Detail:
Title:  Protoplasma     Volume:  -     ISSN:  1615-6102     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Planktologie, ICBM, Carl-von-Ossietzky-Universität Oldenburg, P.O.B. 2503, 26129, Oldenburg, Germany, erhard.rhiel@uni-oldenburg.de.
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