Lectin binding and identification of sialic acid acceptor sugars in rabbit oviduct under hormone administration. | |
MedLine Citation:
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PMID: 8527849 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Localization of individual glycosidic residues and sialic acid acceptor sugars was investigated by conjugated lectins in the rabbit oviduct under physiological hormonal conditions and human chorionic gonadotropin (HCG) administration. Ampulla and isthmus were found to exhibit lectin binding profiles typical of each hormonal stage. Two different sialylated glycomolecules were identified within the epithelial lining; in particular, sialoglycoconjugates characterized by the terminal sequence sialic acid-galactose were visualized in the secretory cells and the sialic acid-N-acetylgalactosamine terminal disaccharides were localized on both ciliated and secretory cells of the entire oviduct. Surface and cytoplasmic sialoglycoconjugates were also found to exhibit a differential behaviour inside the two oviduct tracts examined. Present findings further supported the idea that in ampulla and isthmus, the greatest modifications consequent to hormone treatment take place at different times. |
Authors:
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G Menghi; P Scocco; G Materazzi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microscopy research and technique Volume: 31 ISSN: 1059-910X ISO Abbreviation: Microsc. Res. Tech. Publication Date: 1995 Aug |
Date Detail:
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Created Date: 1996-01-30 Completed Date: 1996-01-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9203012 Medline TA: Microsc Res Tech Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 488-96 Citation Subset: IM |
Affiliation:
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Dipartimento di Biologia M.C.A, Università di Camerino, (MC), Italy. |
Export Citation:
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MeSH Terms | |
Descriptor/Qualifier:
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Acetylgalactosamine
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analysis* Animals Chorionic Gonadotropin / administration & dosage* Epithelium / metabolism Fallopian Tubes / metabolism* Female Galactose / analysis* Horseradish Peroxidase Lectins / metabolism* Rabbits Sialic Acids / analysis* Sialoglycoproteins / metabolism* |
Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 0/Lectins; 0/Sialic Acids; 0/Sialoglycoproteins; 26566-61-0/Galactose; 31022-50-1/Acetylgalactosamine; EC 1.11.1.-/Horseradish Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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