| Learning-induced arg 3.1/arc mRNA expression in the mouse brain. | |
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MedLine Citation:
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PMID: 12663748 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effector immediate-early gene (IEG) arg 3.1, also called arc, encodes a protein interacting with the neuronal cytoskeleton. The selective localization of arg 3.1/arc mRNA in activated dendritic segments suggests that the arg 3.1/arc protein may be synthesized at activated post-synaptic sites and that arg 3.1/arc could participate in structural and functional modifications underlying cognitive processes like memory formation. To analyze whether learning itself is sufficient to trigger expression of arg 3.1/arc, we developed a one-trial learning paradigm in which mice learned to enter a dark compartment to escape from an aversively illuminated area. Arg 3.1/arc mRNA expression was analyzed by in situ hybridization in three groups of mice as follows: a control group with no access to the dark compartment, a learning group having access to the dark compartment for one trial, and a retrieval group having access to the dark compartment for two trials on consecutive days. All animals from the learning and retrieval groups escaped the illuminated area, and those tested 24 h later (retrieval group) showed a strongly reduced latency to enter the dark compartment, demonstrating the validity of our learning paradigm to induce long-term memory. Our results show that acquisition of a simple task results in a brain area-specific biphasic increase in arg 3.1/arc mRNA expression 15 min and 4.5 h post-training. This increase was detected specifically in the learning group but neither in the control nor in the retrieval groups. The pattern of arg 3.1/arc mRNA expression corresponds temporally to the two mRNA- and protein-synthesis-dependent periods of long-term memory formation. Our study provides the first unequivocal evidence that arg 3.1/arc expression is induced by a learning task and strongly suggests a role of arg 3.1/arc mRNA in the early and late cellular mechanisms underlying the stabilization of the memory trace. |
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Authors:
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Monique Montag-Sallaz; Dirk Montag |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Learning & memory (Cold Spring Harbor, N.Y.) Volume: 10 ISSN: 1072-0502 ISO Abbreviation: Learn. Mem. Publication Date: 2003 Mar-Apr |
Date Detail:
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Created Date: 2003-03-28 Completed Date: 2003-07-25 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9435678 Medline TA: Learn Mem Country: United States |
Other Details:
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Languages: eng Pagination: 99-107 Citation Subset: IM |
Affiliation:
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Neurogenetics Research Group, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany. sallaz@ifn-magdeburg.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / metabolism*, physiology Brain Mapping Cytoskeletal Proteins / metabolism*, physiology Gene Expression Regulation In Situ Hybridization Learning* Male Memory* Mice Mice, Inbred C57BL Nerve Tissue Proteins / metabolism*, physiology Neurons / metabolism, physiology RNA, Messenger / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cytoskeletal Proteins; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/activity regulated cytoskeletal-associated protein |
| Comments/Corrections | |
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