Document Detail


Learning-induced arg 3.1/arc mRNA expression in the mouse brain.
MedLine Citation:
PMID:  12663748     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effector immediate-early gene (IEG) arg 3.1, also called arc, encodes a protein interacting with the neuronal cytoskeleton. The selective localization of arg 3.1/arc mRNA in activated dendritic segments suggests that the arg 3.1/arc protein may be synthesized at activated post-synaptic sites and that arg 3.1/arc could participate in structural and functional modifications underlying cognitive processes like memory formation. To analyze whether learning itself is sufficient to trigger expression of arg 3.1/arc, we developed a one-trial learning paradigm in which mice learned to enter a dark compartment to escape from an aversively illuminated area. Arg 3.1/arc mRNA expression was analyzed by in situ hybridization in three groups of mice as follows: a control group with no access to the dark compartment, a learning group having access to the dark compartment for one trial, and a retrieval group having access to the dark compartment for two trials on consecutive days. All animals from the learning and retrieval groups escaped the illuminated area, and those tested 24 h later (retrieval group) showed a strongly reduced latency to enter the dark compartment, demonstrating the validity of our learning paradigm to induce long-term memory. Our results show that acquisition of a simple task results in a brain area-specific biphasic increase in arg 3.1/arc mRNA expression 15 min and 4.5 h post-training. This increase was detected specifically in the learning group but neither in the control nor in the retrieval groups. The pattern of arg 3.1/arc mRNA expression corresponds temporally to the two mRNA- and protein-synthesis-dependent periods of long-term memory formation. Our study provides the first unequivocal evidence that arg 3.1/arc expression is induced by a learning task and strongly suggests a role of arg 3.1/arc mRNA in the early and late cellular mechanisms underlying the stabilization of the memory trace.
Authors:
Monique Montag-Sallaz; Dirk Montag
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Learning & memory (Cold Spring Harbor, N.Y.)     Volume:  10     ISSN:  1072-0502     ISO Abbreviation:  Learn. Mem.     Publication Date:    2003 Mar-Apr
Date Detail:
Created Date:  2003-03-28     Completed Date:  2003-07-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9435678     Medline TA:  Learn Mem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  99-107     Citation Subset:  IM    
Affiliation:
Neurogenetics Research Group, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany. sallaz@ifn-magdeburg.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / metabolism*,  physiology
Brain Mapping
Cytoskeletal Proteins / metabolism*,  physiology
Gene Expression Regulation
In Situ Hybridization
Learning*
Male
Memory*
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins / metabolism*,  physiology
Neurons / metabolism,  physiology
RNA, Messenger / metabolism
Chemical
Reg. No./Substance:
0/Cytoskeletal Proteins; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/activity regulated cytoskeletal-associated protein
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