Document Detail

Lead inhibits secretion of osteonectin/SPARC without significantly altering collagen or Hsp47 production in osteoblast-like ROS 17/2.8 cells.
MedLine Citation:
PMID:  1412468     Owner:  NLM     Status:  MEDLINE    
In an effort to better understand the consequences of lead (Pb2+) on skeletal growth, the effects of Pb2+ were investigated using ROS 17/2.8 bone-like cells in vitro. These studies revealed that Pb2+ (4.5 x 10(-6) M -4.5 x 10(-7) M) has little or no effect on cell shape except when added immediately following seeding of the cells. However, proliferation of ROS cells was inhibited, in the absence of serum, at concentrations of 4.5 x 10(-6) M Pb2+. Protein production was generally increased, however, the major structural protein of bone, type I collagen, production was only slightly altered. Following treatment of ROS cells with Pb2+, intracellular levels of the calcium-binding protein osteonectin/SPARC were increased. Osteonectin/SPARC secretion into the media was delayed or inhibited. Coincident with retention of osteonectin/SPARC there was a decrease in the levels of osteonectin/SPARC mRNA as determined by Northern analysis. These studies suggest that processes associated with osteonectin/SPARC translation and secretion are sensitive to Pb2+.
J J Sauk; T Smith; E K Silbergeld; B A Fowler; M J Somerman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  116     ISSN:  0041-008X     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-11-19     Completed Date:  1992-11-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  240-7     Citation Subset:  IM    
Department of Pathology, University of Maryland Dental School, Baltimore 21201.
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MeSH Terms
Cell Adhesion / drug effects
Cell Division / drug effects
Collagen / biosynthesis*
Endoplasmic Reticulum / metabolism
Heat-Shock Proteins / biosynthesis*
Lead / toxicity*
Organometallic Compounds / administration & dosage
Osteoblasts / drug effects*,  metabolism,  secretion
Osteonectin / secretion*
Osteosarcoma / metabolism,  secretion
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Heat-Shock Proteins; 0/Organometallic Compounds; 0/Osteonectin; 301-04-2/lead acetate; 7439-92-1/Lead; 9007-34-5/Collagen

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