| Lead inhibits in vitro creatine kinase and pyruvate kinase activity in brain cortex of rats. | |
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MedLine Citation:
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PMID: 19925858 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lead intoxication is a serious occupational disease that constitutes a major public health problem. Lead, a heavy metal, has been used by humans for many technological purposes, which is the main reason for its widespread distribution. The toxic mechanisms of lead on the molecular machinery of living organisms include metal transport, energy metabolism, diverse enzymatic processes, genetic regulation, and membrane ionic channels and signaling molecules. Since lead is able to cross the blood-brain barrier it may cause neurotoxicity. Creatine kinase and pyruvate kinase are two thiol-containing enzymes that exert a key role for cellular energy homeostasis in brain. Our main objective was to investigate the in vitro effect of lead on pyruvate kinase and creatine kinase activities of extracts and subcellular fractions from the brain cortex of rats in the presence or not of thiol-protecting substances such as glutathione and cysteamine. The results showed that lead inhibited the two enzyme activities and the thiol-protecting substances prevented their inhibition. These results suggest that lead inhibits creatine kinase and pyruvate kinase activity by interaction with their thiol groups. Therefore, lead may disrupt energy homeostasis and this effect may contribute to the neurological dysfunction found in lead exposed individuals. |
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Authors:
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Tatiana Wannmacher Lepper; Evandro Oliveira; Gustavo Duarte Waltereith Koch; Daiane Bolzan Berlese; Luciane Rosa Feksa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-16 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 24 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-09 Completed Date: 2010-06-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 1045-51 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
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Departamento de Bioqu?mica, Instituto de Ci?ncias B?sicas da Sa?de, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cerebral Cortex / drug effects, enzymology* Creatine Kinase / antagonists & inhibitors* Cysteamine / pharmacology Cytosol / drug effects, enzymology Dose-Response Relationship, Drug Enzyme Inhibitors* Glutathione / pharmacology Lead / toxicity* Lead Poisoning, Nervous System / enzymology* Male Mitochondria / drug effects, enzymology Protective Agents / pharmacology Pyruvate Kinase / antagonists & inhibitors* Rats Rats, Wistar Subcellular Fractions / drug effects, enzymology Sulfhydryl Compounds / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Protective Agents; 0/Sulfhydryl Compounds; 60-23-1/Cysteamine; 70-18-8/Glutathione; 7439-92-1/Lead; EC 2.7.1.40/Pyruvate Kinase; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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