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Lead-induced Nitric Oxide Generation Plays A Critical Role In Lead Uptake Of Pogonatherum crinitum Root Cells.
MedLine Citation:
PMID:  22904111     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Effects of lead (Pb) on endogenous nitric oxide (NO) generation, role of NO in Pb uptake and origin of Pb-induced NO production in Pogonatherum crinitum root cells were evaluated. Pb treatment induced rapid NO generation, showing that Pb exposure triggered endogenous NO signaling of the cells. Pretreatment of the cells with the NO-specific scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline -1-oxyl-3-oxide (cPTIO) not only abolished Pb-triggered NO burst but also reduced Pb contents of the cells. Moreover, Pb exposure enhanced nitrate reductase (NR) activity of the cells. NR inhibitors tungstate and glutamine (Gln) not only suppressed the Pb-enhanced NR activities but also reduced the Pb-triggered NO generation. Pretreatment of the cells with tungstate and Gln suppressed Pb accumulation and the suppression could be restored by application of exogenous NO via its donor sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO). Together, our results indicated that Pb exposure enhanced NR activity and triggered NO burst of P. crinitum root cells. Furthermore, the data demonstrated that NR was responsible for Pb-triggered NO burst and that NR-mediated NO generation played a critical role in Pb uptake of P. crinitum root cells. Thus, our results suggest a potential strategy for controlling Pb uptake of plants by targeting NR as a source of Pb-triggered NO production.
Authors:
Qin Yu; Lian Sun; Haihong Jin; Qian Chen; Zunwei Chen; Maojun Xu
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-17
Journal Detail:
Title:  Plant & cell physiology     Volume:  -     ISSN:  1471-9053     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310035, China.
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