Document Detail


Lateral phase separation in interfacial films of pulmonary surfactant.
MedLine Citation:
PMID:  8913596     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine if lateral phase separation occurs in films of pulmonary surfactant, we used epifluorescence microscopy and Brewster angle microscopy (BAM) to study spread films of calf lung surfactant extract (CLSE). Both microscopic methods demonstrated that compression produced domains of liquid-condensed lipids surrounded by a liquid-expanded film. The temperature dependence of the pressure at which domains first emerged for CLSE paralleled the behavior of its most prevalent component, dipalmitoyl phosphatidylcholine (DPPC), although the domains appeared at pressures 8-10 mN/m higher than for DPPC over the range of 20-37 degrees C. The total area occupied by the domains at room temperature increased to a maximum value at 35 mN/m during compression. The area of domains reached 25 +/- 5% of the interface, which corresponds to the predicted area of DPPC in the monolayer. At pressures above 35 mN/m, however, both epifluorescence and BAM showed that the area of the domains decreased dramatically. These studies therefore demonstrate a pressure-dependent gap in the miscibility of surfactant constituents. The monolayers separate into two phases during compression but remain largely miscible at higher and lower surface pressures.
Authors:
B M Discher; K M Maloney; W R Schief; D W Grainger; V Vogel; S B Hall
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  71     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-03-03     Completed Date:  1997-03-03     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2583-90     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Oregon Health Sciences University, Portland 97201, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biophysical Phenomena
Biophysics
Cattle
Hydrostatic Pressure
Lung / chemistry
Microscopy / methods
Microscopy, Fluorescence / methods
Pulmonary Surfactants / chemistry*
Solubility
Surface Properties
Grant Support
ID/Acronym/Agency:
GM 08437/GM/NIGMS NIH HHS; HL 03502/HL/NHLBI NIH HHS; HL 54209/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Pulmonary Surfactants
Comments/Corrections

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