Document Detail


Lateral hypothalamic NMDA receptor subunits NR2A and/or NR2B mediate eating: immunochemical/behavioral evidence.
MedLine Citation:
PMID:  10070151     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells within the lateral hypothalamic area (LHA) are important in eating control. Glutamate or its analogs, kainic acid (KA) and N-methyl-D-aspartate (NMDA), elicit intense eating when microinjected there, and, conversely, LHA-administered NMDA receptor antagonists suppress deprivation- and NMDA-elicited eating. The subunit composition of LHA NMDA receptors (NMDA-Rs) mediating feeding, however, has not yet been determined. Identifying this is important, because distinct second messengers/modulators may be activated by NMDA-Rs with differing compositions. To begin to address this, we detected LHA NR2A and NR2B subunits by immunoblotting and NR2B subunits by immunohistochemistry using subunit-specific antibodies. To help determine whether NMDA-Rs mediating feeding might contain these subunits, we conducted behavioral studies using LHA-administered ifenprodil, an antagonist selective for NR2A- and/or NR2B-containing NMDA-Rs at the doses we used (0.001-100 nmol). Ifenprodil maximally suppressed NMDA- and deprivation-elicited feeding by 63 and 39%, respectively, but failed to suppress KA-elicited eating, suggesting its actions were behaviorally specific. Collectively, these results suggest that LHA NMDA-Rs, some of which contribute to feeding control, are composed of NR2A and/or NR2B subunits, and implicate NR2A- and/or NR2B-linked signal transduction in feeding behavior.
Authors:
A M Khan; M C Currás; J Dao; F A Jamal; C A Turkowski; R K Goel; E R Gillard; S D Wolfsohn; B G Stanley
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  276     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-04-15     Completed Date:  1999-04-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R880-91     Citation Subset:  IM    
Affiliation:
Division of Biomedical Sciences, University of California, Riverside, California 92521, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Behavior, Animal / drug effects,  physiology
Blotting, Western
Excitatory Amino Acid Antagonists / pharmacology
Feeding Behavior / drug effects,  physiology*
Food Deprivation / physiology
Hypothalamic Area, Lateral / metabolism*
Immunohistochemistry
Isomerism
Kainic Acid / pharmacology
Male
Piperidines / pharmacology
Rats
Rats, Sprague-Dawley
Receptors, N-Methyl-D-Aspartate / metabolism,  physiology*
Grant Support
ID/Acronym/Agency:
NS-24268/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Excitatory Amino Acid Antagonists; 0/Piperidines; 0/Receptors, N-Methyl-D-Aspartate; 23210-56-2/ifenprodil; 487-79-6/Kainic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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