| Lateral diffusion of inositol 1,4,5-trisphosphate receptor type 1 in Purkinje cells is regulated by calcium and actin filaments. | |
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MedLine Citation:
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PMID: 20626556 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inositol 1,4,5-trisphosphate receptor type 1 (IP(3) R1) is an intracellular Ca(2+) release channel that plays crucial roles in the functions of Purkinje cells. The dynamics of IP(3) R1 on the endoplasmic reticulum membrane and the distribution of IP(3) R1 in neurons are thought to be important for the spatial regulation of Ca(2+) release. In this study, we analyzed the lateral diffusion of IP(3) R1 in Purkinje cells in cerebellar slice cultures using fluorescence recovery after photobleaching. In the dendrites of Purkinje cells, IP(3) R1 showed lateral diffusion with an effective diffusion constant of approximately 0.30 μm(2) /s, and the diffusion of IP(3) R1 was negatively regulated by actin filaments. We found that actin filaments were also involved in the regulation of IP(3) R1 diffusion in the spine of Purkinje cells. Glutamate or quisqualic acid stimulation, which activates glutamate receptors and leads to a Ca(2+) transient in Purkinje cells, decreased the diffusion of IP(3) R1 and increased the density of actin in spines. These findings indicate that the neuronal activity-dependent augmentation of actin contributes to the stabilization of IP(3) R1 in spines. |
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Authors:
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Kazumi Fukatsu; Hiroko Bannai; Takafumi Inoue; Katsuhiko Mikoshiba |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-19 |
Journal Detail:
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Title: Journal of neurochemistry Volume: 114 ISSN: 1471-4159 ISO Abbreviation: J. Neurochem. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-24 Completed Date: 2010-10-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: England |
Other Details:
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Languages: eng Pagination: 1720-33 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal Compilation © 2010 International Society for Neurochemistry. |
Affiliation:
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Laboratory for Developmental Neurobiology, Brain Science Institute RIKEN, Wako, Saitama, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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physiology* Animals Calcium / physiology* Dendrites / drug effects, metabolism* Dendritic Spines / drug effects, metabolism Diffusion Excitatory Amino Acid Agonists / pharmacology Fluorescence Recovery After Photobleaching Glutamic Acid / pharmacology Green Fluorescent Proteins / genetics Inositol 1,4,5-Trisphosphate Receptors / genetics, metabolism* Mice Mice, Inbred ICR Microfilaments / physiology* Purkinje Cells / drug effects, metabolism*, ultrastructure Quisqualic Acid / pharmacology Receptors, Glutamate / physiology Tissue Culture Techniques |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Excitatory Amino Acid Agonists; 0/Inositol 1,4,5-Trisphosphate Receptors; 0/Receptors, Glutamate; 147336-22-9/Green Fluorescent Proteins; 52809-07-1/Quisqualic Acid; 56-86-0/Glutamic Acid; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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