Document Detail

Lateral asymmetry of constitutive heterochromatin in human chromosomes.
MedLine Citation:
PMID:  527977     Owner:  NLM     Status:  MEDLINE    
Variations in lateral asymmetry of constitutive heterochromatin were studied in 30 normal individuals with reference to the chromosomal regions 1q12, 9q12, 15p11, 16q12 and Yq12. The technique consisted of growing human lymphocytes for one cell cycle in BrdU, staining with 33258 Hoechst, exposing them to UV light, treating them with 2 x SSC, and staining with Giemsa. This procedure revealed asymmetric staining in the region of constitutive heterochromatin in these chromosomal regions. Chromosomes 15, 16, and Y showed simple lateral asymmetry, whereas chromosome 1 showed both simple and compound asymmetry. In 15 cases, compound lateral asymmetry was evident in both homologues of chromosome 1, 12 cases showed compound lateral asymmetry in one homologue and simple lateral asymmetry in both the homologues. The centromere region of chromosome 9 stained symmetrically with this technique. The lateral asymmetry is presumed to reflect the strand bias in the distribution of thymine in satellite DNA fractions.
P K Ghosh; R Rani; R Nand
Related Documents :
62717 - Chromosomal considerations--application of modern cytogenetics.
24611447 - Xy females do better than the xx in the african pygmy mouse, mus minutoides.
12035617 - Asymmetric staining of ctenomys chromosomes after treatment with alui restriction nucle...
10743817 - Expression of vitronectin and its integrin receptors in the synovial membrane-like inte...
1380697 - A ribosomal ambiguity mutation in the 530 loop of e. coli 16s rrna.
19123967 - Changes in the frequency of ym versus iiim in the housefly, musca domestica l., under f...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human genetics     Volume:  52     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1979 Nov 
Date Detail:
Created Date:  1980-04-17     Completed Date:  1980-04-17     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  79-84     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Centromere / ultrastructure
Chromosome Banding
Chromosomes, Human / ultrastructure*
DNA, Satellite / analysis
Heterochromatin / ultrastructure*
Lymphocytes / ultrastructure
Thymine / analysis
Reg. No./Substance:
0/DNA, Satellite; 0/Heterochromatin; 59-14-3/Bromodeoxyuridine; 65-71-4/Thymine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  X pentasomy: a case and review.
Next Document:  Partial trisomy 4q: two cases resulting from a familial translocation t(4;18)(q27;p11).