Document Detail


Lateral asymmetry of constitutive heterochromatin in human chromosomes.
MedLine Citation:
PMID:  527977     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Variations in lateral asymmetry of constitutive heterochromatin were studied in 30 normal individuals with reference to the chromosomal regions 1q12, 9q12, 15p11, 16q12 and Yq12. The technique consisted of growing human lymphocytes for one cell cycle in BrdU, staining with 33258 Hoechst, exposing them to UV light, treating them with 2 x SSC, and staining with Giemsa. This procedure revealed asymmetric staining in the region of constitutive heterochromatin in these chromosomal regions. Chromosomes 15, 16, and Y showed simple lateral asymmetry, whereas chromosome 1 showed both simple and compound asymmetry. In 15 cases, compound lateral asymmetry was evident in both homologues of chromosome 1, 12 cases showed compound lateral asymmetry in one homologue and simple lateral asymmetry in both the homologues. The centromere region of chromosome 9 stained symmetrically with this technique. The lateral asymmetry is presumed to reflect the strand bias in the distribution of thymine in satellite DNA fractions.
Authors:
P K Ghosh; R Rani; R Nand
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human genetics     Volume:  52     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1979 Nov 
Date Detail:
Created Date:  1980-04-17     Completed Date:  1980-04-17     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  79-84     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bromodeoxyuridine
Centromere / ultrastructure
Chromosome Banding
Chromosomes, Human / ultrastructure*
DNA, Satellite / analysis
Heterochromatin / ultrastructure*
Humans
Lymphocytes / ultrastructure
Male
Thymine / analysis
Chemical
Reg. No./Substance:
0/DNA, Satellite; 0/Heterochromatin; 59-14-3/Bromodeoxyuridine; 65-71-4/Thymine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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