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Latent TGF-β binding protein 3 identifies a second heart field in zebrafish.
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MedLine Citation:
PMID:  21623370     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The four-chambered mammalian heart develops from two fields of cardiac progenitor cells distinguished by their spatiotemporal patterns of differentiation and contributions to the definitive heart. The first heart field differentiates earlier in lateral plate mesoderm, generates the linear heart tube and ultimately gives rise to the left ventricle. The second heart field (SHF) differentiates later in pharyngeal mesoderm, elongates the heart tube, and gives rise to the outflow tract and much of the right ventricle. Because hearts in lower vertebrates contain a rudimentary outflow tract but not a right ventricle, the existence and function of SHF-like cells in these species has remained a topic of speculation. Here we provide direct evidence from Cre/Lox-mediated lineage tracing and loss-of-function studies in zebrafish, a lower vertebrate with a single ventricle, that latent TGF-β binding protein 3 (ltbp3) transcripts mark a field of cardiac progenitor cells with defining characteristics of the anterior SHF in mammals. Specifically, ltbp3(+) cells differentiate in pharyngeal mesoderm after formation of the heart tube, elongate the heart tube at the outflow pole, and give rise to three cardiovascular lineages in the outflow tract and myocardium in the distal ventricle. In addition to expressing Ltbp3, a protein that regulates the bioavailability of TGF-β ligands, zebrafish SHF cells co-express nkx2.5, an evolutionarily conserved marker of cardiac progenitor cells in both fields. Embryos devoid of ltbp3 lack the same cardiac structures derived from ltbp3(+) cells due to compromised progenitor proliferation. Furthermore, small-molecule inhibition of TGF-β signalling phenocopies the ltbp3-morphant phenotype whereas expression of a constitutively active TGF-β type I receptor rescues it. Taken together, our findings uncover a requirement for ltbp3-TGF-β signalling during zebrafish SHF development, a process that serves to enlarge the single ventricular chamber in this species.
Authors:
Yong Zhou; Timothy J Cashman; Kathleen R Nevis; Pablo Obregon; Sara A Carney; Yan Liu; Aihua Gu; Christian Mosimann; Samuel Sondalle; Richard E Peterson; Warren Heideman; Caroline E Burns; C Geoffrey Burns
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-05-29
Journal Detail:
Title:  Nature     Volume:  474     ISSN:  1476-4687     ISO Abbreviation:  Nature     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-07-01     Completed Date:  2011-07-11     Revised Date:  2014-04-25    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  England    
Other Details:
Languages:  eng     Pagination:  645-8     Citation Subset:  IM    
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Bank Name/Acc. No.:
GENBANK/JF731042
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MeSH Terms
Descriptor/Qualifier:
Animals
Cardiovascular Abnormalities / embryology
Cell Lineage
Gene Knockdown Techniques
Heart / embryology*
Latent TGF-beta Binding Proteins / metabolism*
Molecular Sequence Data
Myocardium / cytology,  metabolism*
Phenotype
Signal Transduction
Transcription Factors / metabolism
Zebrafish / embryology*,  genetics
Zebrafish Proteins / metabolism
Grant Support
ID/Acronym/Agency:
5R01HL096816/HL/NHLBI NIH HHS; R01 ES012716/ES/NIEHS NIH HHS; R01 ES012716/ES/NIEHS NIH HHS; R01 HL096816-03/HL/NHLBI NIH HHS; T32 GM007205/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Latent TGF-beta Binding Proteins; 0/Nkx2.5 protein, zebrafish; 0/Transcription Factors; 0/Zebrafish Proteins
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-journal-id): 0410462
Journal ID (pubmed-jr-id): 6011
Journal ID (nlm-ta): Nature
Journal ID (iso-abbrev): Nature
ISSN: 0028-0836
ISSN: 1476-4687
Article Information
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nihms-submitted publication date: Day: 26 Month: 3 Year: 2012
Electronic publication date: Day: 29 Month: 5 Year: 2011
pmc-release publication date: Day: 04 Month: 4 Year: 2012
Volume: 474 Issue: 7353
First Page: 645 Last Page: 648
ID: 3319150
PubMed Id: 21623370
DOI: 10.1038/nature10094
ID: nihpa365514

Latent TGFβ binding protein 3 identifies a second heart field in zebrafish
Yong Zhou12
Timothy J. Cashman12
Kathleen R. Nevis12
Pablo Obregon12
Sara A. Carney3
Yan Liu24
Aihua Gu125
Christian Mosimann26
Samuel Sondalle127
Richard E. Peterson3
Warren Heideman3
Caroline E. Burns127*
C. Geoffrey Burns12*
1Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA 02129
2Harvard Medical School, Boston, MA 02115
3Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin, Madison, WI, 53705
4Nephrology Division, Massachusetts General Hospital, Charlestown, MA 02129
5School of Public Health, Nanjing Medical University, Nanjing, 210029, China
6Stem Cell Program and Division of Hematology/Oncology, Children’s Hospital Boston, MA 02115
7Harvard Stem Cell Institute, Cambridge, MA 02138
*Correspondence and requests for materials should be addressed to C.E.B. (cburns6@partners.org) and/or C.G.B. (cburns@crvc.mgh.harvard.edu)

Using in situ hybridization, we discovered that zebrafish ltbp3 transcripts are expressed in cells at the outflow pole of the linear heart tube (Fig. 1a, b and Supplementary Fig. 1 and 2). Double marker analyses confirmed that a majority of ltbp3+ cells are non-overlapping with differentiated myocardium (Fig. 1c–e and Supplementary Fig. 2). Additionally, ltbp3+ cells are neither endothelial nor derived from the neural crest because ltbp3 expression remains robust in embryos lacking both cell types (Supplementary Fig. 3). Because the anatomical relationship of ltbp3+ cells to the heart tube is reminiscent of the anterior segment of the SHF in mice [3, 12], ltbp3+ cells were evaluated for co-expression of nkx2.5, an evolutionarily conserved marker of CPCs in both heart fields [4]. As reported previously, cells in the linear heart tube were positive for nkx2.5 [13]. Surprisingly, nkx2.5 expression also overlapped with ltbp3 transcripts demonstrating that an extra-cardiac population of ltbp3+, nkx2.5+ cells resides at the outflow pole of the zebrafish heart tube (Fig. 1f–h). This population was also readily identified in double transgenic Tg(nkx2.5BAC::ZsYellow); Tg(cmlc2::CSY) embryos expressing ZsYellow and AmCyan proteins from nkx2.5 and myocardial (cmlc2) promoters respectively (Fig. 1o–q). Lastly, these cells co-express zebrafish tgfβ3 transcripts (Supplementary Fig. 4) consistent with the demonstrated function of LTBP proteins as regulators of TGFβ signaling [11].

To determine if Ltbp3 is required for zebrafish cardiogenesis, we evaluated embryos injected with anti-sense ltbp3 morpholinos (MOltbp3; Supplementary Fig. 5) for chamber-specific defects in cardiomyocyte and endocardial cell number. Knocking down ltbp3 halved the number of ventricular cardiomyocytes and endocardial cells, while atrial cell numbers were unaffected (Fig. 2a–f). The ventricular deficit was evident earlier in development, soon after formation of the heart tube, as shortening of the ventricular segment accompanied by a defect in cardiac looping (Fig. 2g–i). MOltbp3 animals also lacked Eln2+ OFT smooth muscle precursor cells (Fig. 2j–m) [7, 14] homologous to SHF-derived smooth muscle surrounding the base of the aorticopulmonary trunk in higher vertebrates [10]. Lastly, ltbp3 morphants failed to form the ventral aorta (data not shown) and a majority of pharyngeal arch arteries (Supplementary Fig. 6). Taken together, these data demonstrate that knocking down ltbp3 causes multi-lineage cardiovascular defects in the pharyngeal arches, OFT, and ventricle.

In mammals, the anterior segment of the SHF gives rise to the embryonic OFT and the right ventricular half of the common embryonic ventricle prior to septation [13]. Despite the fact that zebrafish embryos never septate their single ventricles, the MOltbp3 phenotype is remarkably similar to mouse anterior SHF mutants that die prior to septation with severe reductions in the primitive right ventricle and OFT [1517]. Therefore, we tested the hypothesis that ltbp3+ cells represent a SHF-like population that gives rise to some or all of the structures missing in MOltbp3 embryos. To that end, we used Cre/Lox-mediate lineage tracing to irreversibly mark ltbp3+ cells and their descendents that assume myocardial, endocardial/endothelial, and smooth muscle cell fates. First, we derived a transgenic driver strain, Tg(ltbp3::TagRFP2Acre), that co-expresses a red fluorescent protein (TagRFP) and Cre recombinase in ltbp3+ cells (Fig. 3a and Supplementary Fig. 7). Secondly, we generated three lineage-restricted reporter strains that carry a unique Cre-responsive “color switching” cassette (AmCyan-Switch-ZsYellow; CSY) under transcriptional control of myocardial (cmlc2), endothelial (kdrl), and smooth muscle precursor cell (eln2) [7, 14] promoters (Fig. 3b and Supplementary Fig. 8). We also generated a ubiquitous reporter strain using the zebrafish ubiquitin promoter [18] (Fig. 3b and Supplementary Fig.8). For each reporter, we confirmed Cre-dependent AmCyan to ZsYellow “color switching” caused by excision of the floxed AmCyan-STOP sequence from the reporter cassette (Supplementary Fig. 8).

Double transgenic progeny from the driver and myocardial reporter strains expressed ZsYellow protein in approximately the distal half of the ventricle (Fig. 3c) demonstrating that myocardium in this segment of the ventricle descends from ltbp3+ progenitors. Myocardial cells in the proximal OFT also arise from ltbp3+ cells (Supplementary Fig. 9). Furthermore, MOltbp3 ventricles lacked ltbp3+ cell derived cardiomyocytes confirming that the distal ventricle is specifically affected in morphant embryos (Fig. 3d). In higher vertebrates, SHF cells labeled with a tracking dye after heart tube formation migrate into the OFT tract and ventricle [12, 19, 20]. Similarly, we injected CellTrackerRed into the non-myocardial (AmCyan), nkx2.5+ (ZsYellow+) region of double transgenic Tg(nkx2.5BAC::ZsYellow); Tg(cmlc2::CSY) embryos at the heart tube stage and observed subsequent dye migration into the distal ventricle and OFT (Fig. 3 e–h). Taken together, these data demonstrate that ltbp3+ cells give rise to distal ventricular myocardium through late differentiation and accretion to the heart tube.

By crossing our driver line with the endothelial and smooth muscle reporter strains, we learned that ltbp3-expressing cells also give rise to endothelial and smooth muscle cells in the OFT (Fig. 3i, j). In smooth muscle reporter embryos, ZsYellow fluorescence was predominantly observed at the base of the OFT (Fig. 3j), suggesting that ltbp3+ cells make a regionalized contribution to OFT smooth muscle. We did not observe color switching in ventricular endocardium or pharyngeal arch artery endothelium suggesting that these cellular compartments do not derive from ltbp3+ cells (data not shown). Finally, lineage tracing ltbp3+ cells with the ubiquitous reporter corroborated the conclusions drawn from lineage-restricted reporters (Fig. 3k–n). Taken together, these data demonstrate that ltbp3+ cells, in addition to giving rise to ventricular myocardium, also give rise to three cardiovascular lineages in the OFT.

To elucidate the cellular mechanism(s) underlying the MOltbp3 phenotype, we evaluated MOltbp3 embryos, soon after formation of the heart tube, for cellular defects in the extra-cardiac population of ltbp3+, nkx2.5+ cells at its outflow pole. Although TagRFP-expressing ltbp3+ cells are too faint to visualize in Tg(ltbp3::TagRFP2Acre) embryos at this developmental stage, non-myocardial ZsYellow-expressing nkx2.5+ cells are readily detected in Tg(nkx2.5::ZsYellow); Tg(cmlc2::CSY) embryos (Fig. 1o–q). Shortly after heart tube formation, both control and MOltbp3 embryos harbored extra-cardiac nkx2.5+ cells demonstrating that progenitor specification is not compromised by loss of ltbp3 function (Fig. 3o, p). However, eleven hours later, this population was absent specifically in MOltbp3 animals (Fig. 3q, r). The absence of TUNEL staining suggested strongly that progenitor survival is not compromised (data not shown). By contrast, BrdU staining revealed that progenitor proliferation is significantly reduced in MOltbp3 embryos (Fig. 3s–u). Taken together, these data support a model in which ltbp3 functions in an autocrine fashion to stimulate proliferation or self-renewal of ltbp3+, nkx2.5+, progenitors to sustain adequate CPC numbers during their differentiation and migration from the SHF. Based on this model, we speculate that knocking down ltbp3 causes a premature depletion of CPCs from the SHF.

LTBP proteins are secreted with TGFβ ligands in a large latent complex (LLC) that becomes anchored to the extracellular matrix prior to ligand activation [11]. Because LTBP proteins are also required for secretion of TGFβ ligands, we used three approaches to test the hypothesis that defective TGFβ signaling underlies the cardiovascular phenotypes seen in MOltbp3 embryos. First, we evaluated the linear heart tube and surrounding regions for phosphorylated pSmad2 epitopes, a hallmark of active TGFβ signaling [21]. TGFβ signaling was observed in the heart tube proper and in extra-cardiac cells approximating the location of ltbp3+, nkx2.5+ progenitors (Fig. 4a). Loss of ltbp3 function eliminated pSmad2 epitopes specifically in the extra-cardiac population leaving signaling in the heart tube unaffected. These data demonstrate that morphant embryos exhibit a highly localized defect in TGFβ signaling specifically in the SHF (Fig. 4B). Next, we evaluated whether small molecule inhibition of TGFβ signaling would phenocopy MOltbp3 animals. To that end, we used a selective inhibitor (LY-364947; [22]) of the TGFβ type I receptor ALK5 that effectively abrogated TGFβ signaling in zebrafish embryos (Fig 4c). LY-364947-treated animals phenocopied ltbp3 morphants as evidenced by reductions in ltbp3+ cell derived myocardium and OFT smooth muscle (Fig. 4d–g). Using LY-364947, we also determined the developmental time window, 26–36 hours post-fertilization during which TGFβ signaling is required for the formation of the distal ventricle (Supplementary Fig. 10), the same time window during which nkx2.5+ progenitors disappear in MOltbp3 embryos (Fig. 3o–r). Lastly, we attempted to rescue MOltbp3 embryos by inducing ubiquitous expression of a constitutively active TGFβ-type I receptor (caALK5), the same receptor targeted by LY-364947, predicted to be downstream of ltbp3 and TGFβ ligands (Supplementary Fig. 9). In a majority of embryos, heat-shock inducible expression of caALK5 partially or fully rescued the loss of distal myocardium seen in MOltbp3 embryos (Fig. 4h–j). Taken together, these results suggest that attenuation of TGFβ signaling underlies the cardiovascular phenotypes present in MOltbp3 embryos.

Because ltbp3+ cells differentiate after formation of the heart tube in pharyngeal mesoderm, elongate the tube at the outflow pole, and give rise to three cardiovascular lineages in the OFT and myocardium in the distal ventricle, they exhibit defining characteristics of the mouse anterior SHF. As such, ltbp3+ cells would also be predicted to encompass the zebrafish equivalents of the avian secondary and anterior heart fields [1, 19, 23].

Our findings are consistent with a recent study demonstrating that pre-existing ventricular cardiomyocytes do not give rise to new cardiomyocytes during elongation of the zebrafish heart tube [9]. The same authors report that the zebrafish homolog of Islet1, a well-known marker of mammalian SHF cells, is dispensable for formation of the distal ventricle, herein reported to derive from the zebrafish SHF. Thus, it appears that the critical role of Islet1, during anterior SHF development, has become minimized or replaced by another factor specifically in the fish lineage. Alternatively, Islet1 function during mammalian anterior SHF development might have co-evolved with the four-chambered heart [24].

Although zebrafish do not have a right ventricle, ltbp3+ cells accrete the distal ventricular segment to the zebrafish heart tube much like SHF cells accrete the primitive right ventricle to the mouse heart tube prior to septation. In mouse, the SHF-derived segment becomes a distinct chamber after septation. In zebrafish, the SHF-derived segment instead augments the singular ventricular chamber, a trait that may have conferred an evolutionary advantage to a common ancestor of both zebrafish and mammals.


Methods Summary

Single and double, fluorescent and non-fluorescent, in situ hybridization and immunohistochemical stainings were performed using standard protocols. To analyze the ltbp3 loss of function phenotype, we injected anti-sense ltbp3 morpholinos into one-cell stage WT and transgenic embryos. For genetic lineage tracing, a transgenic driver strain expressing Cre recombinase in ltbp3+ cells and four Cre-responsive “color switching” reporter strains were generated using standard methods. The driver strain was crossed individually to each of the reporter strains and their double transgenic progenies were analyzed for ZsYellow protein fluorescence using confocal microscopy. To follow the migration of zebrafish SHF cells, a tracking dye was injected into the ZsYellow+, AmCyan region of Tg(nkx2.5::ZsYello); Tg(cmlc2::CSY) embryos at 24hpf, the embryos were imaged immediately, and then again at 48 and/or 72hpf. A transgenic strain carrying a cDNA encoding a constitutively active human TGFβ type I receptor (caALK5) under control of the zebrafish heat shock promoter was generated and used to rescue the myocardial defect in ltbp3 morphant embryos.

Full Methods and any associated references are available in the online version of the paper at www.natre.com/nature


Supplementary Material supp info

Notes

FN1Supplementary Information is linked to the online version of the paper at www.nature.com/nature.

FN2Author Contributions Y.Z. performed the majority of the experiments and analyzed data; T.C., K.N., P.O, Y.L., A.G, and S.S. performed experiments and analyzed data; C.M. provided the ubiquitin promoter prior to publication; S.C., R.P., W.H. and C.G.B. discovered that ltbp3 transcripts are upregulated in a cardiac fraction on day 3 post fertilization; C.G.B performed experiments including BAC recombineering; C.E.B. and C.G.B. co-directed the study, analyzed data, and wrote the paper with input from all authors.

FN3Author Information The zebrafish ltbp3 cDNA sequence was deposited into GenBank under accession number JF731042.

FN4Reprints and permissions information is available at www.nature.com/reprints.

FN5The authors declare no competing financial interests.

Acknowledgements

We thank M. Whitman for advice on pSmad2 staining, D. Hami and M. Kirby for providing Eln2 anti-sera and their immunohistochemistry protocol, R. Cornell for providing tfAP2a and tfAP2c morpholinos, M. Whitman, A. Srinivasan, D. Langenau, E. Provost, S. Leach, R. Anderson, D. Stainier, I.Woods, and A. Schier for providing plasmids. J.W. Xiong for providing clom39 fish, B. Barut and L. Zon for providing BACs, and the MGH Nephrology Division for access to their confocal microscopy facilities. S.C., R.E.P., and W.H. were supported by NIH grant R01 ES012716 from the National Institute of Environmental Health Sciences. C.M. received support through an EMBO long-term fellowship and a HFSP long-term fellowship. This work was funded by the Cardiovascular Research Center at Massachusetts General Hospital, a Claflin Distinguished Scholar Award and Harvard Stem Cell Institute Seed Grant to C.E. Burns, and by awards from the National Heart Lung and Blood Institute (5R01HL096816), American Heart Association (Grant in Aid # 10GRNT4270021), and Harvard Stem Cell Institute (Seed Grant) to C.G. Burns.


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