| Late G1 amino acid restriction point in human dermal fibroblasts. | |
| | |
MedLine Citation:
|
PMID: 3900098 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Human dermal fibroblasts arrested in G0 by maintenance in medium supplemented with 0.1% serum were not restimulated to divide when fresh medium containing 10% dialyzed serum but lacking group B amino acids (cystine, isoleucine, lysine, phenylalanine and tyrosine) was added. Unlike rodent cells, the addition of fresh serum-supplemented medium lacking only isoleucine did not cause a growth arrest. The amino acid sensitive growth arrest in human fibroblasts was dependent both on presynchronization in G0 as well as a prestarvation for amino acids prior to stimulation with high serum. When cells were restimulated in the absence of amino acids, they arrested predominantly in G1, although a small percentage of cells entered early S phase. When medium containing a complete complement of amino acids was then added, cells initiated DNA synthesis following a minimum lag of 2-3 hr. Growth arrested cells initiated DNA synthesis even when complete unsupplemented medium was added, although the addition of high concentrations of insulin or 10% serum increased the rate of entry. |
| | |
Authors:
|
J L Cooper; W Wharton |
Related Documents
:
|
7817668 - Dicarboxylic acids affect the growth of dermatophytes in vitro. 12925668 - Determining the role of n remobilization for growth of apple (malus domestica borkh) tr... 3131218 - Formation of some extracellular enzymes during the exponential growth of bacillus subti... 21764298 - A novel mechanism and kinetic model to explain enhanced xylose yields from dilute sulfu... 7466288 - Excretion of bile acids in healthy children and children with cystic fibrosis. 18171348 - Ppar agonists treatment is effective in a nonalcoholic fatty liver disease animal model... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Journal of cellular physiology Volume: 124 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1985 Sep |
Date Detail:
|
Created Date: 1985-11-21 Completed Date: 1985-11-21 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 433-8 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
physiology* Animals Blood Cell Division Fibroblasts / cytology Flow Cytometry Humans Insulin / pharmacology Interphase* Isoleucine / physiology Kinetics Male Mice Mice, Inbred BALB C Skin / cytology* |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 11061-68-0/Insulin; 73-32-5/Isoleucine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Investigation of adenylate energy charge, phosphorylation potential, and ATP concentration in cells ...
Next Document: DNA damage production in CHO cells at elevated temperatures.