Document Detail

Lassa virus nucleoprotein mutants generated by reverse genetics induce a robust type I interferon response in human dendritic cells and macrophages.
MedLine Citation:
PMID:  21880754     Owner:  NLM     Status:  MEDLINE    
Lassa virus (LASV; Arenaviridae) is responsible for severe hemorrhagic fevers in Africa. LASV nucleoprotein (NP) plays important roles in regulating viral transcription and replication and in inhibiting type I interferon (IFN) production. The NP C-terminal domain contains a 3'-to-5' exonuclease activity involved in suppressing IFN induction. We have established a murine polymerase (Pol) I reverse genetics system for LASV, showing that residues D389 and G392 of NP were critical for LASV viability, while the D389A/G392A and D389T/392A double mutants were severely altered in the ability to suppress IFN in macrophages and dendritic cells. Assessing their attenuation in vivo may open new perspectives in vaccinology.
Xavier Carnec; Sylvain Baize; Stéphanie Reynard; Laure Diancourt; Valérie Caro; Noel Tordo; Michèle Bouloy
Related Documents :
12354764 - Specific activation of human interleukin-5 depends on de novo synthesis of an ap-1 comp...
1391934 - Progress in understanding the pathogenesis of the anemia of chronic disease.
18421214 - Cytokine dysregulation in chronic kidney disease: how can we treat it?
1755994 - Lymphohematopoietic cytokines in the female reproductive tract.
19662634 - Bacterial rna is recognized by different sets of immunoreceptors.
25275594 - Microrna-207 enhances radiation-induced apoptosis by directly targeting akt3 in cochlea...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-08-31
Journal Detail:
Title:  Journal of virology     Volume:  85     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-10-24     Completed Date:  2011-12-16     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12093-7     Citation Subset:  IM    
Unité de Génétique Moléculaire des Bunyavirus, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris cedex 15, France.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/FR832710;  FR832711
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Substitution
Capsid Proteins / genetics*,  immunology*
Cercopithecus aethiops
Dendritic Cells / immunology*,  virology
Interferon Type I / secretion*
Lassa virus / genetics*,  growth & development,  immunology*
Macrophages / immunology*,  virology
Molecular Sequence Data
Mutant Proteins / genetics,  immunology
Mutation, Missense
Sequence Analysis, DNA
Vero Cells
Viral Load
Viral Plaque Assay
Reg. No./Substance:
0/Capsid Proteins; 0/Interferon Type I; 0/Mutant Proteins; 0/nucleocapsid protein, lassa virus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Kaposi's sarcoma-associated herpesvirus Rta tetramers make high-affinity interactions with repetitiv...
Next Document:  Annual vaccination against influenza virus hampers development of virus-specific CD8? T cell immunit...