Document Detail


Lassa virus nucleoprotein mutants generated by reverse genetics induce a robust type I interferon response in human dendritic cells and macrophages.
MedLine Citation:
PMID:  21880754     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lassa virus (LASV; Arenaviridae) is responsible for severe hemorrhagic fevers in Africa. LASV nucleoprotein (NP) plays important roles in regulating viral transcription and replication and in inhibiting type I interferon (IFN) production. The NP C-terminal domain contains a 3'-to-5' exonuclease activity involved in suppressing IFN induction. We have established a murine polymerase (Pol) I reverse genetics system for LASV, showing that residues D389 and G392 of NP were critical for LASV viability, while the D389A/G392A and D389T/392A double mutants were severely altered in the ability to suppress IFN in macrophages and dendritic cells. Assessing their attenuation in vivo may open new perspectives in vaccinology.
Authors:
Xavier Carnec; Sylvain Baize; Stéphanie Reynard; Laure Diancourt; Valérie Caro; Noel Tordo; Michèle Bouloy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-08-31
Journal Detail:
Title:  Journal of virology     Volume:  85     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-10-24     Completed Date:  2011-12-16     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12093-7     Citation Subset:  IM    
Affiliation:
Unité de Génétique Moléculaire des Bunyavirus, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris cedex 15, France.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/FR832710;  FR832711
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Substitution
Animals
Capsid Proteins / genetics*,  immunology*
Cercopithecus aethiops
Dendritic Cells / immunology*,  virology
Interferon Type I / secretion*
Lassa virus / genetics*,  growth & development,  immunology*
Macrophages / immunology*,  virology
Molecular Sequence Data
Mutant Proteins / genetics,  immunology
Mutation, Missense
Sequence Analysis, DNA
Vero Cells
Viral Load
Viral Plaque Assay
Chemical
Reg. No./Substance:
0/Capsid Proteins; 0/Interferon Type I; 0/Mutant Proteins; 0/nucleocapsid protein, lassa virus
Comments/Corrections

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