Document Detail


Laser spectroscopic studies of interactions of UVI with bacterial phosphate species.
MedLine Citation:
PMID:  12866546     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have investigated the interactions of UVI with two bacterial phosphate-containing species: Gram-positive Bacillus sphaericus and Gram-negative Pseudomonas aeruginosa. The Gram-positive B. sphaericus was investigated by using Raman spectroscopy and time-resolved laser-induced fluorescence spectroscopy (TRLFS). We found that living cells, spores, and intact heat-killed cells complexed UVI (pH 4.5) through phosphate groups bound to their surfaces, while decomposed cells released H2PO4- and precipitated UVI as UO2(H2PO4)2. TRLFS of UVI showed that Gram-negative P. aeruginosa--genetically engineered to accumulate polyphosphate, subsequently degrade it, and secrete phosphate--precipitated UVI quantitatively at pH 4.5. The same bacterial strain, not induced to secrete phosphate, sorbed only a small amount of UVI.
Authors:
Roger Knopp; Petra J Panak; Lewis A Wray; Neil S Renninger; Jay D Keasling; Heino Nitsche
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Chemistry (Weinheim an der Bergstrasse, Germany)     Volume:  9     ISSN:  0947-6539     ISO Abbreviation:  Chemistry     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-07-16     Completed Date:  2003-10-09     Revised Date:  2009-08-04    
Medline Journal Info:
Nlm Unique ID:  9513783     Medline TA:  Chemistry     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2812-8     Citation Subset:  IM    
Affiliation:
Lawrence Berkeley National Laboratory, Nuclear Science Division, Berkeley, CA 94720, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacillus / metabolism*
Phosphoric Acid Esters / metabolism*
Pseudomonas aeruginosa / metabolism*
Spectrometry, Fluorescence
Spectrum Analysis, Raman
Uranium / metabolism*
Water Microbiology
Chemical
Reg. No./Substance:
0/Phosphoric Acid Esters; 7440-61-1/Uranium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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