Document Detail


Laser scanning cytometry: principles and applications-an update.
MedLine Citation:
PMID:  23027005     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Laser scanning cytometer (LSC) is the microscope-based cytofluorometer that offers a plethora of unique analytical capabilities, not provided by flow cytometry (FCM). This review describes attributes of LSC and covers its numerous applications derived from plentitude of the parameters that can be measured. Among many LSC applications the following are emphasized: (a) assessment of chromatin condensation to identify mitotic, apoptotic cells, or senescent cells; (b) detection of nuclear or mitochondrial translocation of critical factors such as NF-κB, p53, or Bax; (c) semi-automatic scoring of micronuclei in mutagenicity assays; (d) analysis of fluorescence in situ hybridization (FISH) and use of the FISH analysis attribute to measure other punctuate fluorescence patterns such as γH2AX foci or receptor clustering; (e) enumeration and morphometry of nucleoli and other cell organelles; (f) analysis of progeny of individual cells in clonogenicity assay; (g) cell immunophenotyping; (h) imaging, visual examination, or sequential analysis using different probes of the same cells upon their relocation; (i) in situ enzyme kinetics, drug uptake, and other time-resolved processes; (j) analysis of tissue section architecture using fluorescent and chromogenic probes; (k) application for hypocellular samples (needle aspirate, spinal fluid, etc.); and (l) other clinical applications. Advantages and limitations of LSC are discussed and compared with FCM.
Authors:
Piotr Pozarowski; Elena Holden; Zbigniew Darzynkiewicz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  931     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-02     Completed Date:  2013-02-11     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  187-212     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Nucleus / metabolism
Cytoplasm / metabolism
DNA Damage
Fluorescent Dyes / chemistry
Humans
Image Processing, Computer-Assisted
Immunophenotyping
In Situ Hybridization, Fluorescence
Laser Scanning Cytometry*
Micronucleus Tests
Protein Transport
Grant Support
ID/Acronym/Agency:
CA 28704/CA/NCI NIH HHS; R01 CA028704/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes
Comments/Corrections

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