| Laser scanning cytometry for automation of the micronucleus assay. | |
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MedLine Citation:
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PMID: 21164197 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Laser scanning cytometry (LSC) provides a novel approach for automated scoring of micronuclei (MN) in different types of mammalian cells, serving as a biomarker of genotoxicity and mutagenicity. In this review, we discuss the advances to date in measuring MN in cell lines, buccal cells and erythrocytes, describe the advantages and outline potential challenges of this distinctive approach of analysis of nuclear anomalies. The use of multiple laser wavelengths in LSC and the high dynamic range of fluorescence and absorption detection allow simultaneous measurement of multiple cellular and nuclear features such as cytoplasmic area, nuclear area, DNA content and density of nuclei and MN, protein content and density of cytoplasm as well as other features using molecular probes. This high-content analysis approach allows the cells of interest to be identified (e.g. binucleated cells in cytokinesis-blocked cultures) and MN scored specifically in them. MN assays in cell lines (e.g. the CHO cell MN assay) using LSC are increasingly used in routine toxicology screening. More high-content MN assays and the expansion of MN analysis by LSC to other models (i.e. exfoliated cells, dermal cell models, etc.) hold great promise for robust and exciting developments in MN assay automation as a high-content high-throughput analysis procedure. |
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Authors:
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Zbigniew Darzynkiewicz; Piotr Smolewski; Elena Holden; Ed Luther; Mel Henriksen; Maxime François; Wayne Leifert; Michael Fenech |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Review |
Journal Detail:
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Title: Mutagenesis Volume: 26 ISSN: 1464-3804 ISO Abbreviation: Mutagenesis Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-17 Completed Date: 2011-03-25 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 8707812 Medline TA: Mutagenesis Country: England |
Other Details:
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Languages: eng Pagination: 153-61 Citation Subset: IM |
Affiliation:
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Department of Pathology, New York Medical College, Valhalla, NY 10595, USA. darzynk@nymc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals CHO Cells Cricetinae Cricetulus DNA / analysis Erythrocytes / ultrastructure Flow Cytometry / methods* Humans Image Processing, Computer-Assisted / methods* Laser Scanning Cytometry / methods* Micronucleus Tests Mouth Mucosa / ultrastructure |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA028704-30/CA/NCI NIH HHS; R01 CA028704-31/CA/NCI NIH HHS; R01 CA028704-32/CA/NCI NIH HHS; R01 CA28704/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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