Document Detail


Laser scanning cytometry for automation of the micronucleus assay.
MedLine Citation:
PMID:  21164197     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Laser scanning cytometry (LSC) provides a novel approach for automated scoring of micronuclei (MN) in different types of mammalian cells, serving as a biomarker of genotoxicity and mutagenicity. In this review, we discuss the advances to date in measuring MN in cell lines, buccal cells and erythrocytes, describe the advantages and outline potential challenges of this distinctive approach of analysis of nuclear anomalies. The use of multiple laser wavelengths in LSC and the high dynamic range of fluorescence and absorption detection allow simultaneous measurement of multiple cellular and nuclear features such as cytoplasmic area, nuclear area, DNA content and density of nuclei and MN, protein content and density of cytoplasm as well as other features using molecular probes. This high-content analysis approach allows the cells of interest to be identified (e.g. binucleated cells in cytokinesis-blocked cultures) and MN scored specifically in them. MN assays in cell lines (e.g. the CHO cell MN assay) using LSC are increasingly used in routine toxicology screening. More high-content MN assays and the expansion of MN analysis by LSC to other models (i.e. exfoliated cells, dermal cell models, etc.) hold great promise for robust and exciting developments in MN assay automation as a high-content high-throughput analysis procedure.
Authors:
Zbigniew Darzynkiewicz; Piotr Smolewski; Elena Holden; Ed Luther; Mel Henriksen; Maxime François; Wayne Leifert; Michael Fenech
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review    
Journal Detail:
Title:  Mutagenesis     Volume:  26     ISSN:  1464-3804     ISO Abbreviation:  Mutagenesis     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-17     Completed Date:  2011-03-25     Revised Date:  2013-10-28    
Medline Journal Info:
Nlm Unique ID:  8707812     Medline TA:  Mutagenesis     Country:  England    
Other Details:
Languages:  eng     Pagination:  153-61     Citation Subset:  IM    
Affiliation:
Department of Pathology, New York Medical College, Valhalla, NY 10595, USA. darzynk@nymc.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cricetinae
Cricetulus
DNA / analysis
Erythrocytes / ultrastructure
Flow Cytometry / methods*
Humans
Image Processing, Computer-Assisted / methods*
Laser Scanning Cytometry / methods*
Micronucleus Tests
Mouth Mucosa / ultrastructure
Grant Support
ID/Acronym/Agency:
R01 CA028704/CA/NCI NIH HHS; R01 CA028704-30/CA/NCI NIH HHS; R01 CA028704-31/CA/NCI NIH HHS; R01 CA028704-32/CA/NCI NIH HHS; R01 CA28704/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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