Document Detail


Laser photoacoustic detection allows in planta detection of nitric oxide in tobacco following challenge with avirulent and virulent Pseudomonas syringae Pathovars.
MedLine Citation:
PMID:  16009999     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We demonstrate the use of laser photoacoustic detection (LPAD) as a highly sensitive method to detect in planta nitric oxide ((*)NO) production from tobacco (Nicotiana tabacum). LPAD calibration against (*)NO gas demonstrated a linear relationship over 2 orders of magnitude with a detection threshold of <20 pmol h(-1) (1 part per billion volume [ppbv]). The specificity of the photoacoustic signal for (*)NO when adding gas or the (*)NO donor, sodium nitroprusside, on injection into plant leaves, was demonstrated by its abolition with O(3) ((*)NO + O(3) --> NO(2) + O(2)). The utility of the LPAD method was shown by examination of a nonhost hypersensitive response and a disease induced by Pseudomonas syringae (P. s.) pv phaseolicola and P. s. pv tabaci in tobacco. (*)NO was detected within 40 min of challenge with P. s. pv phaseolicola, some 5 h before the initiation of visible tissue collapse. The wildfire tobacco pathogen P. s. pv tabaci initiated (*)NO generation at 2 h postinfection. The use of (*)NO donors, the scavenger CPTIO ([4-carboxyphenyl]-4,5-dihydro-4,4,5,5-tetramethyl-3-oxide), and the mammalian nitric oxide synthase inhibitor l-NMMA (N(G)-monomethyl-l-arginine) indicated that (*)NO influenced the kinetics of cell death and resistance to both avirulent and virulent bacteria in tobacco. These observations suggest that (*)NO is integral to the elicitation of cell death associated with these two bacterial pathogens in tobacco.
Authors:
Luis A J Mur; I Edi Santosa; Lucas J J Laarhoven; Nicholas J Holton; Frans J M Harren; Aileen R Smith
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant physiology     Volume:  138     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-12     Completed Date:  2005-10-27     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1247-58     Citation Subset:  IM    
Affiliation:
Institute of Biological Sciences, University of Wales, Aberystwyth, SY23 3DA, Wales, United Kingdom. lum@aber.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Acoustics
Carbon Dioxide / metabolism
Ethylenes / metabolism
Lasers*
Light
Nitric Oxide / metabolism*
Plant Diseases / microbiology
Pseudomonas syringae / growth & development,  pathogenicity*
Tobacco / metabolism*,  parasitology*
Virulence
Chemical
Reg. No./Substance:
0/Ethylenes; 10102-43-9/Nitric Oxide; 124-38-9/Carbon Dioxide; 91GW059KN7/ethylene
Comments/Corrections

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