Document Detail


Laser-patterned stem-cell bridges in a cardiac muscle model for on-chip electrical conductivity analyses.
MedLine Citation:
PMID:  22170399     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Following myocardial infarction there is an irreversible loss of cardiomyocytes that results in the alteration of electrical propagation in the heart. Restoration of functional electrical properties of the damaged heart muscle is essential to recover from the infarction. While there are a few reports that demonstrate that fibroblasts can form junctions that transmit electrical signals, a potential alternative using the injection of stem cells has emerged as a promising cellular therapy; however, stem-cell electrical conductivity within the cardiac muscle fiber is unknown. In this study, an in vitro cardiac muscle model was established on an MEA-based biochip with multiple cardiomyocytes that mimic cardiac tissue structure. Using a laser beam, stem cells were inserted adjacent to each muscle fiber (cell bridge model) and allowed to form cell-cell contact as determined by the formation of gap junctions. The electrical conductivity of stem cells was assessed and compared with the electrical conductivities of cardiomyocytes and fibroblasts. Results showed that stem cell-myocyte contacts exhibited higher and more stable conduction velocities than myocyte-fibroblast contacts, which indicated that stem cells have higher electrical compatibility with native cardiac muscle fibers than cardiac fibroblasts.
Authors:
Zhen Ma; Qiuying Liu; Honghai Liu; Huaxiao Yang; Julie X Yun; Carol Eisenberg; Thomas K Borg; Meifeng Xu; Bruce Z Gao
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-12-15
Journal Detail:
Title:  Lab on a chip     Volume:  12     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-11     Completed Date:  2012-05-03     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  566-73     Citation Subset:  IM    
Affiliation:
Department of Bioengineering, Clemson University, Clemson, South Carolina, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Electric Conductivity*
Equipment Design
Fibroblasts / cytology,  pathology
Gap Junctions / metabolism
Lab-On-A-Chip Devices
Microfluidic Analytical Techniques / instrumentation*,  methods*
Models, Biological
Myocardial Infarction / pathology,  therapy
Myocardium / cytology*
Myocytes, Cardiac / cytology,  pathology
Rats
Stem Cells / cytology,  metabolism*
Grant Support
ID/Acronym/Agency:
1K25HL088262-04/HL/NHLBI NIH HHS; K25 HL088262/HL/NHLBI NIH HHS; P20 RR021949/RR/NCRR NIH HHS; P20RR021949/RR/NCRR NIH HHS; R01 HL083236/HL/NHLBI NIH HHS; R01 HL105176/HL/NHLBI NIH HHS
Comments/Corrections

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