Document Detail

Laser nanothermolysis of human leukemia cells using functionalized plasmonic nanoparticles.
MedLine Citation:
PMID:  22720194     Owner:  NLM     Status:  Publisher    
In the present work, we present the use of gold nanorods as plasmonic nanoparticles for selective photothermal therapy of human acute (HL-60) and chronicle (K-562) leukemia cells using a near-infrared laser. We improved a published methodology of gold nanorods conjugation to generate high yields of narrow band gold nanorods with an optical absorption centered at 760 nm. The manufactured nanorods were pegylated and conjugated with monoclonal antibody to become non-toxic as biocompatible nanothermolysis agent. Gold nanorods are synthesized and conjugated to CD33 monoclonal antibody. After pegylation, or conjugation with CD33 antibody, gold nanorods were non-toxic to acute and chronic leukemia cells. Our modified gold nanorods CD33 conjugates shown high level of accumulation for both leukemia cell lines, and successful used for nanothermolysis of human leukemia cells in vitro. Each sample was illuminated with 1 or 3 laser shots as for low and for high laser fluence. The radiation was provided by a Quanta Systems q-switched titanium sapphire laser, and the system was designed for maximum sample coverage using non-focused illumination. HL-60 and K-562 cells were treated for 45 min with gold nanorods CD33 conjugated, or with pegylated gold nanorods. The effect of pulsed-laser nanothermolysis for acute and chronic leukemia cells were investigated with cell counting for number of living cells, percentage of cell death and functional parameters such as damage of cell membrane and metabolic activity. Gold nanorods CD33 conjugates significantly increase cell damage for low fluence laser and completely destroyed cancer cells after 3 pulses for low fluence (acute leukemia) and for high fluence laser as for HL-60 (acute) and for K-562 (chronicle) leukemia cells.
Anton V Liopo; André Conjusteau; Marina Konopleva; Michael Andreeff; Alexander A Oraevsky
Related Documents :
2265454 - Porfiromycin disposition in oxygen-modulated p388 cells.
2470504 - Modification of the metabolism and cytotoxicity of bioreductive alkylating agents by di...
20732244 - Cytotoxicity study of 32 meic chemicals by colony formation and atp assays.
19726054 - Evaluation of toxic side effects of clinically used skin antiseptics in vitro.
7768504 - Alteration of xanthine oxidase activity in sinusoidal endothelial cells and morphologic...
14701674 - Long-term treatment of anterior pituitary cells with nitric oxide induces programmed ce...
Publication Detail:
Journal Detail:
Title:  Nano biomedicine and engineering     Volume:  4     ISSN:  2150-5578     ISO Abbreviation:  -     Publication Date:  2012  
Date Detail:
Created Date:  2012-6-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101531283     Medline TA:  Nano Biomed Eng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  66-75     Citation Subset:  -    
TomoWave Laboratories, 6550 Mapleridge St., Suite 124, Houston TX 77081, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Longitudinal Pathogenesis Study of Young Red Deer (Cervus elaphus) after Experimental Challenge with...
Next Document:  The p38 MAP Kinase Family as Regulators of Proinflammatory Cytokine Production in Degenerative Disea...