Document Detail


Laser microdissection of cells and isolation of high-quality RNA after cryosectioning.
MedLine Citation:
PMID:  22589126     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Laser capture microdissection (LCM) has become a powerful technique that allows analyzing gene expression in specific target cells from complex tissues. It is widely used in animal research, yet few studies on plants have been carried out. We have applied this technique to the plants-nematode interaction by isolating feeding cells (giant cells; GCs) immersed inside complex swelled root structures (galls) induced by root-knot nematodes. For this purpose, a protocol that combines good morphology preservation with RNA integrity maintenance was developed, and successfully applied to Arabidopsis and tomato galls. Specifically, early developing GCs at 3 and 7 days post infection (dpi) were analyzed; RNA from LCM GCs was amplified and used successfully for microarray assays.
Authors:
Marta Barcala; Carmen Fenoll; Carolina Escobar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  883     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  87-95     Citation Subset:  IM    
Affiliation:
Facultad de Ciencias del Medio Ambiente, Universidad de Castilla-La Mancha, Toledo, Spain.
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