| Laser capture microdissection and mRNA characterization of mouse airway epithelium: methodological considerations. | |
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MedLine Citation:
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PMID: 15003609 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The use of laser capture microdissection (LCM) to obtain epithelial cells lining the distal airways for gene profiling is described. In the mouse, the distal airways are particularly attractive for LCM as there is very high percentage of a single cell type, Clara cells, lining these airways. It is shown that the RNA from distal airway epithelial cells harvested by LCM is well preserved and that with linear amplification sufficient cRNA for microarray analysis can be attained from small numbers of cells. |
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Authors:
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Tomoko Betsuyaku; Robert M Senior |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Micron (Oxford, England : 1993) Volume: 35 ISSN: 0968-4328 ISO Abbreviation: Micron Publication Date: 2004 |
Date Detail:
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Created Date: 2004-03-08 Completed Date: 2004-05-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9312850 Medline TA: Micron Country: England |
Other Details:
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Languages: eng Pagination: 229-34 Citation Subset: IM |
Affiliation:
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First Department of Medicine, Hokkaido University School of Medicine, Sapporo, Japan. bytomoko@med.hokudai.ac.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bronchi / cytology, metabolism Cell Separation / methods Epithelial Cells / cytology, metabolism Gene Expression Profiling Lasers Lung / cytology*, metabolism* Mice Microdissection / methods* Microscopy, Electron, Scanning Oligonucleotide Array Sequence Analysis Pulmonary Alveoli / cytology, metabolism RNA, Messenger / genetics*, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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HL 29594/HL/NHLBI NIH HHS; HL 47328/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger |
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