Document Detail


Laser capture microdissection and mRNA characterization of mouse airway epithelium: methodological considerations.
MedLine Citation:
PMID:  15003609     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of laser capture microdissection (LCM) to obtain epithelial cells lining the distal airways for gene profiling is described. In the mouse, the distal airways are particularly attractive for LCM as there is very high percentage of a single cell type, Clara cells, lining these airways. It is shown that the RNA from distal airway epithelial cells harvested by LCM is well preserved and that with linear amplification sufficient cRNA for microarray analysis can be attained from small numbers of cells.
Authors:
Tomoko Betsuyaku; Robert M Senior
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Micron (Oxford, England : 1993)     Volume:  35     ISSN:  0968-4328     ISO Abbreviation:  Micron     Publication Date:  2004  
Date Detail:
Created Date:  2004-03-08     Completed Date:  2004-05-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9312850     Medline TA:  Micron     Country:  England    
Other Details:
Languages:  eng     Pagination:  229-34     Citation Subset:  IM    
Affiliation:
First Department of Medicine, Hokkaido University School of Medicine, Sapporo, Japan. bytomoko@med.hokudai.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Bronchi / cytology,  metabolism
Cell Separation / methods
Epithelial Cells / cytology,  metabolism
Gene Expression Profiling
Lasers
Lung / cytology*,  metabolism*
Mice
Microdissection / methods*
Microscopy, Electron, Scanning
Oligonucleotide Array Sequence Analysis
Pulmonary Alveoli / cytology,  metabolism
RNA, Messenger / genetics*,  metabolism*
Grant Support
ID/Acronym/Agency:
HL 29594/HL/NHLBI NIH HHS; HL 47328/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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